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https://www.arca.fiocruz.br/handle/icict/33006
Tipo de documento
ArtigoDireito Autoral
Acesso restrito
Data de embargo
2022-01-01
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- IOC - Artigos de Periódicos [12500]
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COMPARISON OF VIRAL ELUTION-CONCENTRATION METHODS FOR RECOVERING NOROVIRUSES FROM DELI MEATS
Métodos de concentração de eluição viral
Presunto
Produzir segurança
Carne de peru
Reação em Cadeia da Polimerase em Tempo Real
Viral elution-concentration methods
RT-qPCR
Ham
Turkey meat
Produce safety
Afiliação
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ. Brasil.
Resumo em Inglês
This study aimed to assess viral elution-concentration methods for recovering noroviruses from deli meats.
Spiking experiments were conducted to evaluate the recovery success rates and recovery efficiencies of human
norovirus (NoV) GI and GII and murine norovirus 1 (MNV-1) using polyethylene glycol (PEG 6000) precipitation,
skimmed milk flocculation (SMF), TRIzol® reagent, and a combination of PEG/TRIzol® and SMF/TRIzol®
methods. Molecular analysis using reverse transcriptase quantitative polymerase chain reaction (RT-qPCR) revealed
TRIzol® as the best method to be used for viral recovery from ham with medium recovery rates of 37.6%
for NoV GI and 50.1% for NoV GII. Viral recovery from turkey meat showed medium recovery rates of 14.4% for
NoV GI and 8.9% for NoV GII. For MNV-1, the rates varied from 0.5% to 80.8% not only according to the matrix
but also with the associated virus and its inoculum (NoV GI or GII). The monitoring of commercial samples
obtained in the Great Metropolitan region of Rio de Janeiro in order to demonstrate the occurrence of NoV GI
and GII contamination in both matrices was also performed in 60 samples. NoV GI or GII were not detected in
any samples, while MNV-1 used as the sample process control viruswas successfully recovered in 100% of
samples.
Palavras-chave
NorovirusMétodos de concentração de eluição viral
Presunto
Produzir segurança
Carne de peru
Reação em Cadeia da Polimerase em Tempo Real
Palavras-chave em inglês
NorovirusViral elution-concentration methods
RT-qPCR
Ham
Turkey meat
Produce safety
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