Author | Luz, Isabelle da Silva | |
Author | Miagostovich, Marize Pereira | |
Access date | 2019-05-09T12:26:52Z | |
Available date | 2019-05-09T12:26:52Z | |
Document date | 2018 | |
Citation | LUZ, Isabelle da Silva; MIAGOSTOVICH, Marise Pereira. Comparison of viral elution-concentration methods for recovering noroviruses from deli meats. Journal of Virological Methods, v. 260, p. 49–55, 2018. | pt_BR |
ISSN | 0166-0934 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/33006 | |
Language | eng | pt_BR |
Publisher | Elsevier | pt_BR |
Rights | restricted access | pt_BR |
Subject in Portuguese | Norovirus | pt_BR |
Subject in Portuguese | Métodos de concentração de eluição viral | pt_BR |
Subject in Portuguese | Presunto | pt_BR |
Subject in Portuguese | Produzir segurança | pt_BR |
Subject in Portuguese | Carne de peru | pt_BR |
Subject in Portuguese | Reação em Cadeia da Polimerase em Tempo Real | pt_BR |
Title | Comparison of viral elution-concentration methods for recovering noroviruses from deli meats | pt_BR |
Type | Article | pt_BR |
DOI | 10.1016/j.jviromet.2018.07.001 | |
Abstract | This study aimed to assess viral elution-concentration methods for recovering noroviruses from deli meats.
Spiking experiments were conducted to evaluate the recovery success rates and recovery efficiencies of human
norovirus (NoV) GI and GII and murine norovirus 1 (MNV-1) using polyethylene glycol (PEG 6000) precipitation,
skimmed milk flocculation (SMF), TRIzol® reagent, and a combination of PEG/TRIzol® and SMF/TRIzol®
methods. Molecular analysis using reverse transcriptase quantitative polymerase chain reaction (RT-qPCR) revealed
TRIzol® as the best method to be used for viral recovery from ham with medium recovery rates of 37.6%
for NoV GI and 50.1% for NoV GII. Viral recovery from turkey meat showed medium recovery rates of 14.4% for
NoV GI and 8.9% for NoV GII. For MNV-1, the rates varied from 0.5% to 80.8% not only according to the matrix
but also with the associated virus and its inoculum (NoV GI or GII). The monitoring of commercial samples
obtained in the Great Metropolitan region of Rio de Janeiro in order to demonstrate the occurrence of NoV GI
and GII contamination in both matrices was also performed in 60 samples. NoV GI or GII were not detected in
any samples, while MNV-1 used as the sample process control viruswas successfully recovered in 100% of
samples. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ. Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ. Brasil. | pt_BR |
Subject | Norovirus | pt_BR |
Subject | Viral elution-concentration methods | pt_BR |
Subject | RT-qPCR | pt_BR |
Subject | Ham | pt_BR |
Subject | Turkey meat | pt_BR |
Subject | Produce safety | pt_BR |
DeCS | Reação em Cadeia da Polimerase em Tempo Real | pt_BR |
e-ISSN | 1879-0984 | |
Embargo date | 2022-01-01 | |