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CARDIOMYOGENIC DIFFERENTIATION IS FINE-TUNED BY DIFFERENTIAL MRNA ASSOCIATION WITH POLYSOMES
Cardiomyocytes
Polyribosomes
Gene Expression Regulation
RNA Processing, Post-Transcriptional
Polirribosomas
Regulación de la Expresión Génica
Procesamiento Postranscripcional del ARN
Polirribossomos
Regulação da Expressão Gênica
Processamento Pós-Transcricional do RNA
Autor
Afiliación
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil.
Institut Pasteur. Bioinformatics Unit. Montevideo, Uruguay.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil.
Institut Pasteur. Bioinformatics Unit. Montevideo, Uruguay.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil.
Institut Pasteur. Bioinformatics Unit. Montevideo, Uruguay.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil.
Institut Pasteur. Bioinformatics Unit. Montevideo, Uruguay.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil.
Institut Pasteur. Bioinformatics Unit. Montevideo, Uruguay.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil.
Institut Pasteur. Bioinformatics Unit. Montevideo, Uruguay.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil.
Resumen en ingles
Cardiac cell fate specification occurs through progressive steps, and its gene expression regulation features are still being defined. There has been an increasing interest in understanding the coordination between transcription and post-transcriptional regulation during the differentiation processes. Here, we took advantage of the polysome profiling technique to isolate and high-throughput sequence ribosome-free and polysome-bound RNAs during cardiomyogenesis. We showed that polysome-bound RNAs exhibit the cardiomyogenic commitment gene expression and that mesoderm-to-cardiac progenitor stages are strongly regulated. Additionally, we compared ribosome-free and polysome-bound RNAs and found that the post-transcriptional regulation vastly contributes to cardiac phenotype determination, including RNA recruitment to and dissociation from ribosomes. Moreover, we found that protein synthesis is decreased in cardiomyocytes compared to human embryonic stem-cells (hESCs), possibly due to the down-regulation of translation-related genes. Our data provided a powerful tool to investigate genes potentially controlled by post-transcriptional mechanisms during the cardiac differentiation of hESC. This work could prospect fundamental tools to develop new therapy and research approaches.
Palabras clave en ingles
CardiomyogenesisCardiomyocytes
Polyribosomes
Gene Expression Regulation
RNA Processing, Post-Transcriptional
Palabras clave
Miocitos CardíacosPolirribosomas
Regulación de la Expresión Génica
Procesamiento Postranscripcional del ARN
DeCS
Miócitos CardíacosPolirribossomos
Regulação da Expressão Gênica
Processamento Pós-Transcricional do RNA
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