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ArtículoDerechos de autor
Acceso abierto
Fecha del embargo
2020-05-29
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- INI - Artigos de Periódicos [3466]
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MOLECULAR EPIDEMIOLOGY AND ANTIFUNGAL SUSCEPTIBILITY PATTERNS OF SPOROTHRIX SCHENCKII ISOLATES FROM A CAT-TRANSMITTED EPIDEMIC OF SPOROTRICHOSIS IN RIO DE JANEIRO, BRAZIL
In vitro susceptibility
PCR fingerprinting
Clinical forms
ITS region
Autor
Afiliación
Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Serviço de Infectologia. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Serviço de Micologia. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Serviço de Infectologia. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Serviço de Micologia. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Serviço de Micologia. Rio de Janeiro, RJ, Brasil.
Instituto de Salud Carlos III. Centro Nacional de Microbiología. Servicio de Micología. Madrid, Spain.
Instituto de Salud Carlos III. Centro Nacional de Microbiología. Servicio de Micología. Madrid, Spain.
Instituto de Salud Carlos III. Centro Nacional de Microbiología. Servicio de Micología. Madrid, Spain.
Instituto de Salud Carlos III. Centro Nacional de Microbiología. Servicio de Micología. Madrid, Spain.
Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Serviço de Micologia. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Serviço de Infectologia. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Serviço de Micologia. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Serviço de Micologia. Rio de Janeiro, RJ, Brasil.
Instituto de Salud Carlos III. Centro Nacional de Microbiología. Servicio de Micología. Madrid, Spain.
Instituto de Salud Carlos III. Centro Nacional de Microbiología. Servicio de Micología. Madrid, Spain.
Instituto de Salud Carlos III. Centro Nacional de Microbiología. Servicio de Micología. Madrid, Spain.
Instituto de Salud Carlos III. Centro Nacional de Microbiología. Servicio de Micología. Madrid, Spain.
Resumen en ingles
Since 1998 a cat-transmitted epidemic of sporotrichosis has been observed in Rio de Janeiro (RJ), Brazil. Besides the lymphocutaneous and fixed forms, other presentations, such as disseminated cutaneous and mucosal involvement, as well as for the first time, erythema nodosum and erythema multiforme have been reported associated with sporotrichosis. This study investigates the phenotypes and genotypes of Sporothrix schenckii isolates recovered from different clinical forms of the disease noted as part of this epidemic. A total of 88 isolates recovered from 59 cases associated with the epidemic and 29 controls (from cases in other Brazilian regions and Spain) were included in this study. In vitro susceptibility testing was conducted as part of the phenotypic analysis, while the genotypic analysis involved a DNA fingerprinting method with primer M13 and ribosomal DNA sequencing of the internal transcribed spacer (ITS). MIC values of amphotericin B, itraconazole, posaconazole, ravuconazole, and terbinafine were found to be significantly lower (P<0.01) for isolates associated with the epidemic than for control strains. No differences in MIC values were observed related to clinical forms of the infection. Fingerprinting analysis showed that RJ epidemic strains were genetically related. Although nine subtypes were found, they were not associated with specific clinical forms. Similar results were obtained with the ITS sequence analysis. These data suggest that the strains isolated from the epidemic cases of sporotrichosis in RJ all originated from a common source.
Palabras clave en ingles
Sporothrix schenckiiIn vitro susceptibility
PCR fingerprinting
Clinical forms
ITS region
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