Author | Almeida, Elza Carmen de | |
Author | Martinez, Alejandra Nóbrega | |
Author | Maniero, Viviane Câmara | |
Author | Sales, Ana Maria | |
Author | Duppre, Nádia C. | |
Author | Sarno, Euzenir Nunes | |
Author | Santos, Adalberto Rezende | |
Author | Moraes, Milton Ozório | |
Access date | 2019-05-30T11:43:58Z | |
Available date | 2019-05-30T11:43:58Z | |
Document date | 2004 | |
Citation | ALMEIDA, Elza Carmen de et al. Detection of Mycobacterium leprae DNA by Polymerase Chain Reaction in the Blood and Nasal Secretion of Brazilian Household Contacts. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 99, n. 5, p. 509-512, Aug. 2004. | pt_BR |
ISSN | 0074-0276 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/33278 | |
Language | eng | pt_BR |
Publisher | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz | pt_BR |
Rights | open access | |
Subject in Portuguese | Mycobacterium leprae | pt_BR |
Subject in Portuguese | Reação em cadeia da polimerase | pt_BR |
Subject in Portuguese | Contatos saudáveis | pt_BR |
Subject in Portuguese | Diagnóstico precoce | pt_BR |
Title | Detection of Mycobacterium leprae DNA by Polymerase Chain Reaction in the Blood and Nasal Secretion of Brazilian Household Contacts | pt_BR |
Type | Article | pt_BR |
DOI | 10.1590/S0074-02762004000500009 | pt_BR |
Abstract | DNA samples from blood and nasal swabs of 125 healthy household contacts was submitted to amplification by
polymerase chain reaction (PCR) using a Mycobacterium leprae-specific sequence as a target for the detection of
subclinical infection with M. leprae. All samples were submitted to hybridization analysis in order to exclude any
false positive or negative results. Two positive samples were confirmed from blood out of 119 (1.7%) and two
positive samples from nasal secretion out of 120 (1.7%). The analysis of the families with positive individuals
showed that 2.5% (n = 3) of the contacts were relatives of multibacilary patients while 0.8% of the cases (n = 1) had
a paucibacilary as an index case. All positive contacts were followed up and after one year none of them presented
clinical signs of the disease. In spite of the PCR sensitivity to detect the presence of the M. leprae in a subclinical
stage, this molecular approach did not seem to be a valuable tool to screen household contacts, since we determined
a spurious association of the PCR positivity and further development of leprosy. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Medicina Tropical. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Medicina Tropical. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Medicina Tropical. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Medicina Tropical. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Medicina Tropical. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Medicina Tropical. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Medicina Tropical. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Medicina Tropical. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil. | pt_BR |
Subject | Mycobacterium leprae | pt_BR |
Subject | Polymerase chain reaction | pt_BR |
Subject | Healthy contacts | pt_BR |
Subject | Early diagnosis | pt_BR |
e-ISSN | 1678-8060 | |