Autor | Ishida, Kelly | |
Autor | Castro, Rafaela Alves | |
Autor | Torrado, Juan J. | |
Autor | Serrano, Dolores Remedios | |
Autor | Borba-Santos, Luana Pereira | |
Autor | Quintella, Leonardo Pereira | |
Autor | Souza, Wanderley de | |
Autor | Rozental, Sonia | |
Autor | Lopes-Bezerra, Leila M. | |
Data de acesso | 2019-06-12T14:26:32Z | |
Data de disponibilização | 2019-06-12T14:26:32Z | |
Data do publicação | 2018 | |
Citação | ISHIDA, Kelly et al. Efficacy of a poly-aggregated formulation of amphotericin B in treating systemic sporotrichosis caused by Sporothrix brasiliensis. Medical Mycology, v. 56, p. 288-296, 2018. | pt_BR |
ISSN | 1369-3786 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/33471 | |
Idioma | eng | pt_BR |
Editor | Oxford University Press | pt_BR |
Direito Autoral | open access | pt_BR |
Título | Efficacy of a poly-aggregated formulation of amphotericin B in treating systemic sporotrichosis caused by Sporothrix brasiliensis | pt_BR |
Tipo do documento | Article | pt_BR |
DOI | 10.1093/mmy/myx040 | |
Resumo em Inglês | In severe cases of sporotrichosis, it is recommended to use amphotericin B deoxycholate (D-AMB) or its lipid formulations and/or in association with itraconazole (ITC). Our aim was to evaluate the antifungal efficacy of a poly-aggregated amphotericin B (P-AMB), a nonlipid formulation, compared with D-AMB on systemic sporotrichosis caused by Sporothrix brasiliensis. In vitro assays showed that Sporothrix schenckii sensu stricto and S. brasiliensis yeast clinical isolates were susceptible to low concentrations of P-AMB and D-AMB. Although P-AMB presented a higher minimal inhibitory concentration (MIC) compared to D-AMB, its cytotoxic effect on renal cells and erythrocytes was lower. For the in vivo assays, male BALB/c mice were intravenously infected with S. brasiliensis yeasts, and P-AMB or D-AMB was administered 3 days post-infection. The efficacy of five therapeutic regimens was tested: intravenous monotherapy with P-AMB or D-AMB, intravenous pulsed-therapy with P-AMB or D-AMB, and intravenous therapy with P-AMB, followed by oral ITC. These treatments increased murine survival and controlled the fungal burden in the liver, spleen, lungs, and kidneys. However, only D-AMB monotherapy or the pulsed-therapies with D-AMB or P-AMB led to 100% survival of the mice 45 days post-infection; only pulsed administration of D-AMB was able to control the fungal load in all organs 45 days post-infection. Accordingly, the histopathological findings showed reductions in the fungal burden and inflammatory reactions in these treatment regimens. Together, our results suggest that the P-AMB formulation could be considered as an alternative drug to D-AMB for treating disseminated sporotrichosis. | pt_BR |
Afiliação | University of São Paulo. Institute of Biomedical Sciences. Department of Microbiology. Laboratory of Antifungal Chemotherapy. São Paulo, SP, Brazil. | pt_BR |
Afiliação | Rio de Janeiro State University. Department of Cell Biology. Laboratory of Cellular Mycology and Proteomics. Rio de Janeiro, RJ, Brazil. | pt_BR |
Afiliação | Complutense University. Faculty of Pharmacy. Department of Pharmacy and Pharmaceutical Technology. Madrid, Spain. | pt_BR |
Afiliação | Complutense University. Faculty of Pharmacy. Department of Pharmacy and Pharmaceutical Technology. Madrid, Spain. | pt_BR |
Afiliação | Federal University of Rio de Janeiro. Institute of Biophysics Carlos Chagas Filho. Laboratory of Fungal Cell Biology. Rio de Janeiro, RJ, Brazil. | pt_BR |
Afiliação | Rio de Janeiro State University. Faculty of Medical Sciences. Rio de Janeiro, RJ, Brazil. | pt_BR |
Afiliação | Federal University of Rio de Janeiro. Institute of Biophysics Carlos Chagas Filho. Institute of Biophysics Carlos Chagas Filho. Rio de Janeiro, RJ, Brazil. | pt_BR |
Afiliação | Rio de Janeiro State University. Department of Cell Biology. Laboratory of Cellular Mycology and Proteomics. Rio de Janeiro, RJ, Brazil. | pt_BR |
Afiliação | Rio de Janeiro State University. Department of Cell Biology. Laboratory of Cellular Mycology and Proteomics. Rio de Janeiro, RJ, Brazil. | pt_BR |
Palavras-chave em inglês | Sporotrichosis | pt_BR |
Palavras-chave em inglês | Sporothrix brasiliensis | pt_BR |
Palavras-chave em inglês | Poly-aggregated AMB | pt_BR |
Palavras-chave em inglês | Amphotericin B | pt_BR |
Palavras-chave em inglês | Sporotrichosis mouse model | pt_BR |
Palavras-chave em inglês | Antifungal drugs | pt_BR |
e-ISSN | 1460-2709 | |
Data de embargo | 2020-06-12 | |