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Autor | Bensalah, Mona | |
Autor | Klein, Pierre | |
Autor | Riederer, Ingo | |
Autor | Chaouch, Soraya | |
Autor | Muraine, Laura | |
Autor | Savino, Wilson | |
Autor | Butler-Browne, Gillian Sandra | |
Autor | Trollet, Capucine | |
Autor | Mouly, Vincent | |
Autor | Bigot, Anne | |
Fecha de acceso | 2019-07-04T14:58:22Z | |
Fecha de disponibilización | 2019-07-04T14:58:22Z | |
Fecha de publicación | 2019 | |
Referencia | BENSALAH, Mona et al. Combined methods to evaluate human cells in muscle xenografts. Plos One, v. 14, n. 5, p. 1-12, May 2019. | pt_BR |
ISSN | 1932-6203 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/33839 | |
Idioma | eng | pt_BR |
Editor | Public Library of Science | pt_BR |
Derechos de autor | open access | pt_BR |
Palabras clave en Portugués | Métodos combinados | pt_BR |
Palabras clave en Portugués | Xenógrafos de músculos | pt_BR |
Palabras clave en Portugués | Células humanas | pt_BR |
Palabras clave en Portugués | Xenotransplante | pt_BR |
Título | Combined methods to evaluate human cells in muscle xenografts | pt_BR |
Tipo del documento | Article | pt_BR |
DOI | 10.1371/journal.pone.0211522 | |
Resumen en Inglés | Xenotransplantation of human cells into immunodeficient mouse models is a very powerful tool and an essential step for the pre-clinical evaluation of therapeutic cell- and gene- based strategies. Here we describe an optimized protocol combining immunofluorescence and real-time quantitative PCR to both quantify and visualize the fate and localization of human myogenic cells after injection in regenerating muscles of immunodeficient mice. Whereas real-time quantitative PCR-based method provides an accurate quantification of human cells, it does not document their specific localization. The addition of an immunofluorescence approach using human-specific antibodies recognizing engrafted human cells gives information on the localization of the human cells within the host muscle fibres, in the stem cell niche or in the interstitial space. These two combined approaches offer an accurate evaluation of human engraftment including cell number and localization and should provide a gold standard to compare results obtained either using different types of human stem cells or comparing healthy and pathological muscle stem cells between different research laboratories worldwide. | pt_BR |
Afiliación | Sorbonne Université. Myology Research Center. UM76 and INSERM U974. Institut de Myologie. Paris, France. | pt_BR |
Afiliación | Sorbonne Université. Myology Research Center. UM76 and INSERM U974. Institut de Myologie. Paris, France. | pt_BR |
Afiliación | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ. Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Instituto Nacional em Ciência e Tecnologia em Neuroimunomodulação. Rio de Janeiro, RJ, Brasil. | pt_BR |
Afiliación | Sorbonne Université. Myology Research Center. UM76 and INSERM U974. Institut de Myologie. Paris, France. | pt_BR |
Afiliación | Sorbonne Université. Myology Research Center. UM76 and INSERM U974. Institut de Myologie. Paris, France. | pt_BR |
Afiliación | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ. Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Instituto Nacional em Ciência e Tecnologia em Neuroimunomodulação. Rio de Janeiro, RJ, Brasil. | pt_BR |
Afiliación | Sorbonne Université. Myology Research Center. UM76 and INSERM U974. Institut de Myologie. Paris, France. | pt_BR |
Afiliación | Sorbonne Université. Myology Research Center. UM76 and INSERM U974. Institut de Myologie. Paris, France. | pt_BR |
Afiliación | Sorbonne Université. Myology Research Center. UM76 and INSERM U974. Institut de Myologie. Paris, France. | pt_BR |
Afiliación | Sorbonne Université. Myology Research Center. UM76 and INSERM U974. Institut de Myologie. Paris, France. | pt_BR |
Palavras clave en Inglês | Combined methods | pt_BR |
Palavras clave en Inglês | Muscle xenografts | pt_BR |
Palavras clave en Inglês | Human cells | pt_BR |
Palavras clave en Inglês | Xenotransplantation | pt_BR |
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