Autor | Robert, Anny Waloski | |
Autor | Gomes, Felipe Azevedo | |
Autor | Rode, Michele Patricia | |
Autor | Silva, Maiara Marques da | |
Autor | Veleirinho, Maria Beatriz da Rocha | |
Autor | Maraschin, Marcelo | |
Autor | Hayashi, Leila | |
Autor | Calloni, Giordano Wosgrau | |
Autor | Stimamiglio, Marco Augusto | |
Data de acesso | 2019-07-15T17:40:34Z | |
Data de disponibilização | 2019-07-15T17:40:34Z | |
Data do publicação | 2019 | |
Citação | ROBERT, Anny Waloski et al. The skin regeneration potential of a pro-angiogenic secretome from human skin-derived multipotent stromal cells. Journal of Tissue Engineering, v. 10, p. 1-10, 2019. | pt_BR |
ISSN | 2041-7314 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/34110 | |
Idioma | por | pt_BR |
Editor | SAGE Publications | pt_BR |
Direito Autoral | open access | pt_BR |
Palavras-chave | Secretome | pt_BR |
Título | The skin regeneration potential of a pro-angiogenic secretome from human skin-derived multipotent stromal cells | pt_BR |
Tipo do documento | Article | pt_BR |
DOI | 10.1177/2041731419833391 | |
Resumo em Inglês | Multipotent stromal cells stimulate skin regeneration after acute or chronic injuries. However, many stem cell therapy protocols are limited by the elevated number of cells required and poor cell survival after transplantation. Considering that the beneficial effects of multipotent stromal cells on wound healing are typically mediated by paracrine mechanisms, we examined whether the conditioned medium from skin-derived multipotent stromal cells would be beneficial for restoring the skin structure of mice after wounding. A proteomic characterization of skin-derived multipotent stromal cell-conditioned medium was performed, and the angiogenic function of this secretome was investigated in vitro using an endothelial cell tube formation assay. We then applied the skin-derived multipotent stromal cell-conditioned medium directly to full-thickness excisional wounds or embedded it into carrageenan or poly(vinyl alcohol) hydrogels to monitor tissue regeneration in mice. Biological processes related to wound healing and angiogenesis were highlighted by the analysis of the skin-derived multipotent stromal cell secretome, and a pro-angiogenic capacity for promoting tubule-like structures was first confirmed in vitro. Skin wounds treated with skin-derived multipotent stromal cell-conditioned medium also displayed increased angiogenesis, independently of the association of the conditioned medium with hydrogels. However, improvements in wound closure and epidermis or decreased inflammatory cell presence were not observed. Hence, the use of the secretome obtained from human skin-derived multipotent stromal cells may be a potential strategy to aid the natural skin repair of full-thickness lesions mainly based on its pro-angiogenic properties. | pt_BR |
Afiliação | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. | pt_BR |
Afiliação | Universidade Federal de Santa Catarina. Florianópolis, SC, Brasil. | pt_BR |
Afiliação | Universidade Federal de Santa Catarina. Florianópolis, SC, Brasil. | pt_BR |
Afiliação | Universidade Federal de Santa Catarina. Florianópolis, SC, Brasil. | pt_BR |
Afiliação | Universidade Federal de Santa Catarina. Florianópolis, SC, Brasil. | pt_BR |
Afiliação | Universidade Federal de Santa Catarina. Florianópolis, SC, Brasil. | pt_BR |
Afiliação | Universidade Federal de Santa Catarina. Florianópolis, SC, Brasil. | pt_BR |
Afiliação | Universidade Federal de Santa Catarina. Florianópolis, SC, Brasil. | pt_BR |
Afiliação | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. | pt_BR |
Palavras-chave em inglês | Stromal Cells | pt_BR |
Palavras-chave em inglês | Wound Healing | pt_BR |
Palavras-chave em inglês | Physiologic Angiogenesis | pt_BR |
Palavras-chave em espanhol | Células del Estroma | pt_BR |
Palavras-chave em espanhol | Cicatrización de Heridas | pt_BR |
Palavras-chave em espanhol | Angiogénesis fisiológica | pt_BR |
DeCS | Células Estromais | pt_BR |
DeCS | Cicatrização | pt_BR |
DeCS | Angiogênese Fisiológica | pt_BR |
e-ISSN | 2041-7314 | |