| Author | Leony, Leonardo Maia | |
| Author | Freitas, Natália Erdens Maron de | |
| Author | Del-Rei, Rodrigo Pimenta | |
| Author | Carneiro, Claudia Martins | |
| Author | Reis, Alexandre Barbosa | |
| Author | Jansen, Ana Maria | |
| Author | Xavier, Samanta Cristina das Chagas | |
| Author | Gomes, Yara M. | |
| Author | Silva, Edmilson D. | |
| Author | Reis, Mitermayer Galvão dos | |
| Author | Fraga, Deborah Bittencourt Mothé | |
| Author | Celedon, Paola Alejandra Fiorani | |
| Author | Zanchin, Nilson I. T. | |
| Author | Torres, Filipe Dantas | |
| Author | Santos, Fred Luciano Neves | |
| Access date | 2019-07-31T17:04:53Z | |
| Available date | 2019-07-31T17:04:53Z | |
| Document date | 2019 | |
| Citation | LEONY, Leonardo Maia et al. Performance of recombinant chimeric proteins in the serological diagnosis of Trypanosoma cruzi infection in dogs. Plos Neglected Tropical Diseases, v. 13, n. 6, p. 1-16, 2019. | pt_BR |
| ISSN | 1935-2727 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/34541 | |
| Sponsorship | Gonc¸alo
Moniz Institute (Research Excellence Program -
PROEP/IGM, Grant: 400904/2013-6; Internal
Research Support Program - PIAP Jovem+, Grant:
PIAP-001/2017), and in part by the Coordination of
Superior Level Staff Improvement - Brazil (CAPES,
Grant PROEX 0720/2018). Brazilian National
Council for Scientific and Technological Development (CNPq) and Research Support
Foundation for the State of Rio de Janeiro
(FAPERJ). MGR, NITZ, CMC, ABR and AMJ are
research fellows of CNPq. This study was funded
by Oswaldo Cruz Foundation- FIOCRUZ; Vice-
Presidency of Research and Reference
Laboratories (VPPLR/FIOCRUZ) for investing in our
research; the European Union Seventh Framework
Program Grant 223034 – ChagasEpiNet. | pt_BR |
| Language | eng | pt_BR |
| Publisher | Public Library of Science | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Trypanosoma cruzi | pt_BR |
| Subject in Portuguese | Proteinas quiméricas | pt_BR |
| Subject in Portuguese | Fatores de risco | pt_BR |
| Subject in Portuguese | Transmissão | pt_BR |
| Subject in Portuguese | Cães | pt_BR |
| Subject in Portuguese | Diagnóstico | pt_BR |
| Title | Performance of recombinant chimeric proteins in the serological diagnosis of Trypanosoma cruzi infection in dogs | pt_BR |
| Type | Article | |
| DOI | 10.1371/journal.pntd.0007545 | |
| Abstract | Dogs are considered sentinels in areas of Trypanosoma cruzi transmission risk to humans. ELISA is generally the method of choice for diagnosing T. cruzi exposure in dogs, but its performance substantially depends on the antigenic matrix employed. In previous studies, our group has developed four chimeric antigens (IBMP-8.1, 8.2, 8.3, and 8.4) and evaluated their potential for diagnosing T. cruzi exposure in humans. For human sera, these chimeric antigens presented superior diagnostic performances as compared to commercial tests available in Brazil, Spain, and Argentina. Therefore, in this study we have evaluated the potential of these antigenic proteins for detection of anti-T. cruzi IgG antibodies in dog sera. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Faculty of Technology and Sciences of Bahia. Salvador, BA, Brazil. | pt_BR |
| Affilliation | Federal University of Ouro Preto. Nucleus of Research in Biological Sciences. Immunopathology Laboratory. Ouro Preto, MG, Brazil. | pt_BR |
| Affilliation | Federal University of Ouro Preto. Nucleus of Research in Biological Sciences. Immunopathology Laboratory. Ouro Preto, MG, Brazil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil. | pt_BR |
| Affilliation | Immunobiological Technology Institute. Rio de Janeiro, RJ, Brazil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Federal University of Bahia. Department of Pathology and Legal Medicine. Salvador, BA, Brazil / Yale University. School of Public Health. Department of Epidemiology of Microbial Diseases. New Haven, Connecticut, United States of America. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Molecular Biology Institute of Paraná. Curitiba, PR, Brazil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. | pt_BR |
| Subject | Trypanosoma cruzi | pt_BR |
| Subject | Chimeric Proteins | pt_BR |
| Subject | Risk factors | pt_BR |
| Subject | Transmission | pt_BR |
| Subject | Dogs | pt_BR |
| Subject | Diagnosis | pt_BR |
