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- INI - Artigos de Periódicos [3391]
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COMPARATIVE GENOME ANALYSIS OF PHYLLOSTICTA CITRICARPA AND PHYLLOSTICTA CAPITALENSIS, TWO FUNGI SPECIES THAT SHARE THE SAME HOST
Endophytic species
Citrus black spot
Functional enrichment analysis
Phylogeny
Gene family expansion
Autor(es)
Afiliação
Centro APTA Citros Sylvio Moreira. Laboratório de Biotecnologia. Cordeirópolis, SP, Brasil.
Centro APTA Citros Sylvio Moreira. Laboratório de Biotecnologia. Cordeirópolis, SP, Brasil.
Universidade Estadual de Campinas. Laboratório de Genômica e BioEnergia. Campinas, SP, Brasil.
Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em DST/AIDS. Rio de Janeiro, RJ, Brasil.
Centro APTA Citros Sylvio Moreira. Laboratório de Biotecnologia. Cordeirópolis, SP, Brasil.
Centro APTA Citros Sylvio Moreira. Laboratório de Biotecnologia. Cordeirópolis, SP, Brasil.
Universidade Estadual de Campinas. Laboratório de Genômica e BioEnergia. Campinas, SP, Brasil.
Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em DST/AIDS. Rio de Janeiro, RJ, Brasil.
Centro APTA Citros Sylvio Moreira. Laboratório de Biotecnologia. Cordeirópolis, SP, Brasil.
Resumo em Inglês
Background: Citrus are among the most important crops in the world. However, there are many diseases that
affect Citrus caused by different pathogens. Citrus also hosts many symbiotic microorganisms in a relationship that may be advantageous for both organisms. The fungi Phyllosticta citricarpa, responsible for citrus black spot, and Phyllosticta capitalensis, an endophytic species, are examples of closely related species with different behavior in citrus. Both species are always biologically associated and are morphologically very similar, and comparing their genomes could help understanding the different lifestyles. In this study, a comparison was carried to identify genetic differences that could help us to understand the biology of P. citricarpa and P. capitalensis. Results: Drafts genomes were assembled with sizes close to 33 Mb for both fungi, carrying 15,206 and 14,797 coding sequences for P. citricarpa and P. capitalensis, respectively. Even though the functional categories of these coding sequences is similar, enrichment analysis showed that the pathogenic species presents growth and development genes that may be necessary for the pathogenicity of P. citricarpa. On the other hand, family expansion analyses showed the plasticity of the genome of these species. Particular families are expanded in the genome of an ancestor of P. capitalensis and a recent expansion can also be detected among this species. Additionally, evolution could be driven by environmental cues in P. citricarpa. Conclusions: This work demonstrated genomic differences between P. citricarpa and P. capitalensis. Although the idea that these differences could explain the different lifestyles of these fungi, we were not able to confirm this hypothesis. Genome evolution seems to be of real importance among the Phyllosticta isolates and it is leading to different biological characteristics of these species.
Palavras-chave em inglês
Pathogenic speciesEndophytic species
Citrus black spot
Functional enrichment analysis
Phylogeny
Gene family expansion
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