Author | Iñiguez, Alena Mayo | |
Author | Araújo, Adauto José Goncalves de | |
Author | Silva, Luiz Fernando Rocha Ferreira da | |
Author | Vicente, Ana Carolina Paulo | |
Access date | 2019-08-19T12:58:56Z | |
Available date | 2019-08-19T12:58:56Z | |
Document date | 2003 | |
Citation | IÑIGUEZ, Alena M et al. Analysis of ancient DNA from coprolites: a perspective with random amplified polymorphic DNA-polymerase chain reaction approach. Memórias do Instituto Oswaldo Cruz, v. 98, suplem. 1, p. 1-3, 2003. | pt_BR |
ISSN | 0074-0276 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/34869 | |
Language | eng | pt_BR |
Publisher | Instituto Oswaldo Cruz | pt_BR |
Rights | open access | pt_BR |
Title | Analysis of ancient DNA from coprolites: a perspective with randon amplified polymorphic DNA-polymerase chain reaction approach | pt_BR |
Type | Article | pt_BR |
DOI | 10.1590/S0074-02762003000900012 | |
Abstract | The aim of this work was to determine approaches that would improve the quality of ancient DNA (aDNA) present in coprolites to enhance the possibility of success in retrieving specific sequence targets. We worked with coprolites from South American archaeological sites in Brazil and Chile dating up to 7,000 years ago. Using established protocols for aDNA extraction we obtained samples showing high degradation as usually happens with this kind of material. The reconstructive polymerization pretreatment was essential to overcome the DNA degradation and the serial dilutions helped with to prevent polymerase chain reaction (PCR) inhibitors. Moreover, the random amplified polymorphic DNA-PCR has been shown to be a reliable technique for further experiments to recover specific aDNA sequences. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Genética. Laboratório de Genética Molecular de Microorganismos. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Escola Nacional de Saúde Pública Sergio Arouca. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Escola Nacional de Saúde Pública Sergio Arouca. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Genética. Laboratório de Genética Molecular de Microorganismos. Rio de Janeiro, RJ, Brasil. | pt_BR |
Subject | Molecular Paleoparasitology | pt_BR |
Subject | Coprolites | pt_BR |
Subject | Random Amplified Polymorphic DNA | pt_BR |
Subject | Ancient DNA | pt_BR |
Subject | Polymerase Chain Reaction | pt_BR |
e-ISSN | 1678-8060 | |