Author | Bozzi, Adriana | |
Author | Sayed, Nazish | |
Author | Matsa, Elena | |
Author | Sass, Gabriele | |
Author | Neofytou, Evgenios | |
Author | Clemons, Karl V. | |
Author | Oliveira, Rodrigo Corrêa de | |
Author | Stevens, David A. | |
Author | Wu, Joseph C | |
Access date | 2019-08-21T14:07:21Z | |
Available date | 2019-08-21T14:07:21Z | |
Document date | 2019 | |
Citation | BOZZI, Adriana et al. Using Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes as a Model to Study Trypanosoma cruzi Infection. Stem Cell Reports, v. 12, n. 6, p. 1232-1241, 2019 | pt_BR |
ISSN | 2213-6711 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/34923 | |
Language | eng | pt_BR |
Publisher | Cell Press | pt_BR |
Rights | open access | pt_BR |
Title | Using Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes as a Model to Study Trypanosoma cruzi Infection | pt_BR |
Type | Article | pt_BR |
DOI | 10.1016/j.stemcr.2019.04.017 | |
Abstract | Chagas disease (ChD) is one of the most neglected tropical diseases, with cardiomyopathy being the main cause of death in Trypanosoma cruzi-infected patients. As the parasite actively replicates in cardiomyocytes (CMs), the heart remains a key target organ in the pathogenesis of ChD. Here we modeled ChD using human induced pluripotent stem cell-derived CMs (iPSC-CMs) to understand the complex interplay between the parasite and host cells. We showed that iPSC-CMs can get infected with the T. cruzi Y strain and that all parasite cycle stages can be identified in our model system. Importantly, characterization of T. cruzi-infected iPSC-CMs showed significant changes in their gene expression profile, cell contractility, and distribution of key cardiac markers. Moreover, these infected iPSC-CMs exhibited a pro-inflammatory profile as indicated by significantly elevated cytokine levels and cell-trafficking regulators. We believe our iPSC-CM model is a valuable platform to explore new treatment strategies for ChD. | pt_BR |
Affilliation | Stanford Cardiovascular Institute. Stanford, CA, USA/Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil | pt_BR |
Affilliation | Stanford Cardiovascular Institute. Stanford, CA, USA/Department of Medicine. Division of Cardiovascular Medicine. Stanford University. School of Medicine., Stanford, CA, USA/Department of Radiology. Stanford University. School of Medicine. Stanford, CA, USA | pt_BR |
Affilliation | Stanford Cardiovascular Institute. Stanford, CA, USA/Department of Medicine. Division of Cardiovascular Medicine. Stanford University. School of Medicine., Stanford, CA, USA/Department of Radiology. Stanford University. School of Medicine. Stanford, CA, USA | pt_BR |
Affilliation | Division of Infectious Diseases and Geographic Medicine. Stanford University. School of Medicine, Stanford, CA, USA/California Institute for Medical Research. San Jose, CA, USA | pt_BR |
Affilliation | Stanford Cardiovascular Institute. Stanford, CA, USA/Department of Medicine. Division of Cardiovascular Medicine. Stanford University. School of Medicine., Stanford, CA, USA/Department of Radiology. Stanford University. School of Medicine. Stanford, CA, USA | pt_BR |
Affilliation | Division of Infectious Diseases and Geographic Medicine. Stanford University. School of Medicine, Stanford, CA, USA/California Institute for Medical Research. San Jose, CA, USA | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil | pt_BR |
Affilliation | Division of Infectious Diseases and Geographic Medicine. Stanford University. School of Medicine, Stanford, CA, USA/California Institute for Medical Research. San Jose, CA, USA | pt_BR |
Affilliation | Stanford Cardiovascular Institute. Stanford, CA, USA/Department of Medicine. Division of Cardiovascular Medicine. Stanford University. School of Medicine., Stanford, CA, USA/Department of Radiology. Stanford University. School of Medicine. Stanford, CA, USA | pt_BR |
Subject | induced pluripotent stem cells | pt_BR |
Subject | Trypanosoma cruzi | pt_BR |
Subject | Chagas disease | pt_BR |
Subject | cardiomyopathy | pt_BR |
Subject | disease modeling | pt_BR |
Subject | drug screening | pt_BR |