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https://www.arca.fiocruz.br/handle/icict/35713
PREVIOUS DENGUE OR ZIKA VIRUS EXPOSURE CAN DRIVE TO INFECTION ENHANCEMENT OR NEUTRALISATION OF OTHER FLAVIVIRUSES
Anticorpos virais / imunologia
Reações cruzadas / imunologia
Vírus da dengue / imunologia
Flavivírus / classificação
Flavivírus / imunologia
Citometria de fluxo
Humanos
Testes de neutralização
Vírus Zika / imunologia
Author
Affilliation
Universidade Federal da Paraíba. Departamento de Fisiologia e Patologia. João Pessoa, PB, Brasil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Virologia. Recife, PE, Brasil / Charité-Universitätsmedizin. Berlin. Berlin, Germany.
Universidade Federal da Paraíba. Hospital Universitário Lauro Wanderley. Serviço de Doenças Infecciosas e Parasitárias. João Pessoa, PB, Brasil / Universidade Federal da Paraíba. Escola Técnica de Saúde. Grupo de Estudos e Pesquisas em Imunologia Humana. João Pessoa, PB, Brasil.
Universidade Federal de Pernambuco. Departamento de Medicina Clínica. Recife, PE, Brasil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Virologia. Recife, PE, Brasil / University of Pittsburgh. Center for Vaccine Research. Department of Infectious Diseases and Microbiology. Pittsburgh, PA, USA.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Virologia. Recife, PE, Brasil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Virologia. Recife, PE, Brasil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Virologia. Recife, PE, Brasil / Charité-Universitätsmedizin. Berlin. Berlin, Germany.
Universidade Federal da Paraíba. Hospital Universitário Lauro Wanderley. Serviço de Doenças Infecciosas e Parasitárias. João Pessoa, PB, Brasil / Universidade Federal da Paraíba. Escola Técnica de Saúde. Grupo de Estudos e Pesquisas em Imunologia Humana. João Pessoa, PB, Brasil.
Universidade Federal de Pernambuco. Departamento de Medicina Clínica. Recife, PE, Brasil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Virologia. Recife, PE, Brasil / University of Pittsburgh. Center for Vaccine Research. Department of Infectious Diseases and Microbiology. Pittsburgh, PA, USA.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Virologia. Recife, PE, Brasil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Virologia. Recife, PE, Brasil.
Abstract
BACKGROUND Dengue virus (DENV) has circulated in Brazil for over 30 years. During this time, one serotype has cyclically
replaced the other, until recently, when all four distinct serotypes began to circulate together. Persistent circulation of DENV
for long time periods makes sequential infections throughout a person’s life possible. After primary DENV infection, life-long
immunity is developed for the infecting serotype. Since DENV and Zika virus (ZIKV) are antigenically similar, the possibility
of cross-reactions has attracted attention and has been demonstrated in vitro.
OBJECTIVE The aim of this study was to investigate whether immune-sera from DENV and ZIKV infected patients would crossreact in vitro with other Flaviviridae family members.
METHODS Cross-reaction of the studied samples with yellow fever virus (YFV), West Nile virus (WNV), Rocio virus (ROCV),
Saint Louis virus (SLEV) and Ilheus virus (ILHV) has been investigated by plaque reduction neutralisation test (PRNT) and the
antibody-dependent enhancement (ADE) by flow-cytometry.
FINDINGS Antibodies against ZIKV and DENV virus cross-reacted with other flaviviruses either neutralising or enhancing
the infection. Thus, viral entrance into FcRFcɣRII-expressing cells were influenced by the cross-reactive antibodies. ZIKV or
DENV immune sera enhanced cellular infection by WNV, ILHV, ROCV and SLEV. Finally, DENV immune sera presented
higher neutralising activity for YFV and SLEV. While ZIKV immune sera neutralised WNV, ILHV and ROCV with high
frequencies of positivity.
MAIN CONCLUSIONS The co-circulation of those viruses in the same area represents a risk for the development of severe
infections if they spread throughout the country. Successive flavivirus infections may have an impact on disease pathogenesis,
as well as on the development of safe vaccine strategies.
DeCS
Anticorpos Neutralizadores / imunologiaAnticorpos virais / imunologia
Reações cruzadas / imunologia
Vírus da dengue / imunologia
Flavivírus / classificação
Flavivírus / imunologia
Citometria de fluxo
Humanos
Testes de neutralização
Vírus Zika / imunologia
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