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2022-01-01
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HOST RESISTANCE, GENOMICS AND POPULATION DYNAMICS IN A SALMONELLA ENTERITIDIS AND PHAGE SYSTEM
Salmonella Enteritidis
Bacteria-phage coevolution
Antibiotics
Bacterial resistance
Autor
Afiliación
Department of Biological Sciences. Universidad de Los Andes. Bogotá, Colombia.
Department of Biological Sciences. Universidad de Los Andes. Bogotá, Colombia.
Department of Biological Sciences. Universidad de Los Andes. Bogotá, Colombia.
Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil.
Department of Biological Sciences. Universidad de Los Andes. Bogotá, Colombia.
Department of Biological Sciences. Universidad de Los Andes. Bogotá, Colombia.
Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil / Instituto Tecnológico Vale. Belém, PA, Brazil.
Research Group for Genomic Epidemiology. National Food Institute. Technical University of Denmark. Lyngby, Denmark.
Research Group for Genomic Epidemiology. National Food Institute. Technical University of Denmark. Lyngby, Denmark.
Department of Biological Sciences. Universidad de Los Andes. Bogotá, Colombia.
Department of Biological Sciences. Universidad de Los Andes. Bogotá, Colombia.
Department of Biological Sciences. Universidad de Los Andes. Bogotá, Colombia.
Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil.
Department of Biological Sciences. Universidad de Los Andes. Bogotá, Colombia.
Department of Biological Sciences. Universidad de Los Andes. Bogotá, Colombia.
Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil / Instituto Tecnológico Vale. Belém, PA, Brazil.
Research Group for Genomic Epidemiology. National Food Institute. Technical University of Denmark. Lyngby, Denmark.
Research Group for Genomic Epidemiology. National Food Institute. Technical University of Denmark. Lyngby, Denmark.
Department of Biological Sciences. Universidad de Los Andes. Bogotá, Colombia.
Resumen en ingles
Bacteriophages represent an alternative solution to control bacterial infections. When interacting, bacteria and phage can evolve, and this relationship is described as antagonistic coevolution, a pattern that does not fit all models. In this work, the model consisted of a microcosm of Salmonella enterica serovar Enteritidis and jSan23 phage. Samples were taken for 12 days every 48 h. Bacteria and phage samples were collected; and isolated bacteria from each time point were challenged against phages from previous, contemporary, and subsequent time points. The phage plaque tests, with the genomics analyses, showed a mutational asymmetry dynamic in favor of the bacteria instead of antagonistic coevolution. This is important for future phage-therapy applications, so we decided to explore the population dynamics of Salmonella under different conditions: pressure of one phage, a combination of phages, and phages plus an antibiotic. The data from cultures with single and multiple phages, and antibiotics, were used to create a mathematical model exploring population and resistance dynamics of Salmonella under these treatments, suggesting a nonlethal, growth-inhibiting antibiotic may decrease resistance to phage-therapy cocktails. These data provide a deep insight into bacterial dynamics under different conditions and serve as additional criteria to select phages and antibiotics for phage-therapy.
Palabras clave en ingles
Phage-therapySalmonella Enteritidis
Bacteria-phage coevolution
Antibiotics
Bacterial resistance
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