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https://www.arca.fiocruz.br/handle/icict/36979
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ArtigoDireito Autoral
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Data de embargo
2050-01-01
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MITOCHONDRIAL PERMEABILITY TRANSITION PORE IN SEA URCHIN FEMALE GAMETES
Eggs
Female gametes
Mitochondria
Mitochondrial permeability transition pore
Sea urchin
Cálcio / metabolismo
Ciclosporina / metabolismo
Feminino
Células Germinativas / metabolismo
Potencial da Membrana Mitocondrial / fisiologia
Mitocôndrias / metabolismo
Mitocôndrias / fisiologia
Proteínas de Transporte da Membrana Mitocondrial / metabolismo
Ouriços-do-Mar / metabolismo
Ouriços-do-Mar / fisiologia
Autor(es)
Afiliação
University of Birmingham. Birmingham, United Kingdom.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Microbiologia. Recife, PE, Brasil.
Universidade Federal da Paraíba. Departamento de Biologia Molecular. João Pessoa, PB, Brasil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Microbiologia. Recife, PE, Brasil.
Universidade Federal da Paraíba. Departamento de Biologia Molecular. João Pessoa, PB, Brasil.
Resumo em Inglês
Mitochondrial permeability transition pore (MPTP) has been associated to calcium homeostasis and reactive oxygen species (ROS) generation in several cell types. While extensively investigated in somatic cells, there are few data regarding MPTP phenomenon in gametes. The aim of the present work was to investigate MPTP occurrence in sea urchin female gametes. The protonophores CCCP and FCCP, and the Ca2+ ionophore ionomycin, were used as pore inductors. Pore opening was monitored by mitochondrial potential sensitive probes and cobalt-quenched calcein assay. The pore desensitizer cyclosporin A (CsA) prevented the loss of mitochondrial inner membrane potential (ΔΨm) and pore opening induced by MPTP activators. The disruption of ΔΨm led to an increase in ROS generation, which was completely prevented by CsA. Our data also demonstrated that the increase in ROS production induced by MPTP opening requires extracellular Ca2+. In summary, the current study provides evidence about the occurrence of MPTP in sea urchin eggs in a similar manner as described in vertebrate somatic cells - CsA-sensitive, voltage- and Ca2+-triggered - and shows MPTP as a highly conserved physiological event through the evolution.
Palavras-chave em inglês
CalciumEggs
Female gametes
Mitochondria
Mitochondrial permeability transition pore
Sea urchin
DeCS
AnimaisCálcio / metabolismo
Ciclosporina / metabolismo
Feminino
Células Germinativas / metabolismo
Potencial da Membrana Mitocondrial / fisiologia
Mitocôndrias / metabolismo
Mitocôndrias / fisiologia
Proteínas de Transporte da Membrana Mitocondrial / metabolismo
Ouriços-do-Mar / metabolismo
Ouriços-do-Mar / fisiologia
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