Author | Lisboa, Vinícius da Cunha | |
Author | Ribeiro-Alves, Marcelo | |
Author | Corrêa, Raquel da Silva | |
Author | Lopes, Isabelle Ramos | |
Author | Mafort, Thiago Thomaz | |
Author | Santos, Ana Paula Gomes Dos | |
Author | Amadeu, Thaís Porto | |
Author | Rufino, Rogério | |
Author | Rodrigues, Luciana Silva | |
Access date | 2019-11-18T15:39:44Z | |
Available date | 2019-11-18T15:39:44Z | |
Document date | 2019 | |
Citation | LISBOA, Vinicius da Cunha et al. The predominance of Th1 Immune Response in Pleural Effusion of Patients with Tuberculosis Among Other Exudative Etiologies. Journal of Clinical Microbiology, 2019 | pt_BR |
ISSN | 0095-1137 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/37127 | |
Language | eng | pt_BR |
Publisher | American Society for Microbiology | pt_BR |
Rights | open access | pt_BR |
Title | The predominance of Th1 Immune Response in Pleural Effusion of Patients with Tuberculosis Among Other Exudative Etiologies | pt_BR |
Type | Article | pt_BR |
DOI | 10.1128/JCM.00927-19 | |
Abstract | Pleural tuberculosis (PlTB), a common form of extrapulmonary TB, remains as a challenge in the diagnosis among many causes of pleural effusion. We recently reported that the combinatorial analysis of interferon-gamma (IFN-γ), IFN-γ-inducible protein 10 (IP-10), and adenosine deaminase (ADA) from the pleural microenvironment was useful to distinguish pleural effusion caused by TB (microbiologically or not confirmed cases) among other etiologies. In this cross-sectional cohort study, a set of inflammatory mediators was quantified in blood and pleural fluid (PF) from exudative pleural effusion cases, including PlTB (n = 27) and non-PlTB (nTB; n = 25) patients. The levels of IL-2, IL-4, IL-6, IL-10, IL-17A, IFN-γ, TNF, IP-10, TGF-β1, and ADA were determined using cytometric bead assay, ELISA or biochemical tests. IFN-γ, IP-10, TNF, TGF-β, and ADA quantified in PF showed significantly higher concentrations in PlTB patients when compared to nTB. When blood and PF were compared, we have identified significantly higher concentrations of IL-6 and IL-10 in PF, in both groups. TGF-β, solely, showed significantly increased levels in PF and blood from PlTB when both clinical specimens were compared to nTB patients. Principal components analysis (PCA) revealed a T helper type 1 (Th1) pattern mainly attributed to higher levels of IP-10, IFN-γ, TGF-β, and TNF in pleural cavity, which was distinct between PlTB and nTB. In conclusion, our findings showed a predominantly cellular immune response in PF from TB cases rather than other causes of exudative effusion, commonly considered in the differential diagnosis of PlTB. | pt_BR |
Affilliation | Laboratory of Immunopathology, Medical Science Faculty, Rio de Janeiro State University (UERJ), Rio de Janeiro, RJ, Brazil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em DST/AIDS. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Laboratory of Immunopathology, Medical Science Faculty, Rio de Janeiro State University (UERJ), Rio de Janeiro, RJ, Brazil | pt_BR |
Affilliation | Laboratory of Immunopathology, Medical Science Faculty, Rio de Janeiro State University (UERJ), Rio de Janeiro, RJ, Brazil | pt_BR |
Affilliation | Department of Pulmonary and Tisiology Care, Pedro Ernesto University Hospital (HUPE)/UERJ, Rio de Janeiro, RJ, Brazil. | pt_BR |
Affilliation | Department of Pulmonary and Tisiology Care, Pedro Ernesto University Hospital (HUPE)/UERJ, Rio de Janeiro, RJ, Brazil. | pt_BR |
Affilliation | Laboratory of Immunopathology, Medical Science Faculty, Rio de Janeiro State University (UERJ), Rio de Janeiro, RJ, Brazil. | pt_BR |
Affilliation | Department of Pulmonary and Tisiology Care, Pedro Ernesto University Hospital (HUPE)/UERJ, Rio de Janeiro, RJ, Brazil. | pt_BR |
Affilliation | Laboratory of Immunopathology, Medical Science Faculty, Rio de Janeiro State University (UERJ), Rio de Janeiro, RJ, Brazil. | pt_BR |
Subject | Pleural tuberculosis | pt_BR |
Subject | Pleural effusion | pt_BR |
Subject | Adenosine deaminase | pt_BR |
Subject | Th1 response | pt_BR |
Subject | Cytokines in pleural effusion | pt_BR |
Embargo date | 2020-05-16 | |