Author | Mörking, Patricia Alves | |
Author | Rampazzo, Rita de Cássia Pontello | |
Author | Walrad, Pegine | |
Author | Probst, Christian Macagnan | |
Author | Soares, Maurilio José | |
Author | Gradia, Daniela Fiori | |
Author | Pavoni, Daniela Parada | |
Author | Krieger, Marco Aurélio | |
Author | Matthews, Keith | |
Author | Goldenberg, Samuel | |
Author | Fragoso, Stenio Perdigão | |
Author | Dallagiovanna, Bruno | |
Access date | 2019-12-12T19:46:38Z | |
Available date | 2019-12-12T19:46:38Z | |
Document date | 2012 | |
Citation | MÖRKING, Patrícia Alves et al. The zinc finger protein TcZFP2 binds target mRNAs enriched during Trypanosoma cruzi metacyclogenesis. Memórias do Instituto Oswaldo Cruz. Rio de Janeiro, v. 107, n. 6, p. 790-799, 2012. | pt_BR |
ISSN | 1678-8060 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/37922 | |
Language | eng | pt_BR |
Publisher | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | Trypanosoma cruzi | pt_BR |
Title | The zinc finger protein TcZFP2 binds target mRNAs enriched during Trypanosoma cruzi metacyclogenesis | pt_BR |
Type | Article | pt_BR |
DOI | 10.1590/s0074-02762012000600014 | |
Abstract | Trypanosomes are parasitic protozoa in which gene expression is primarily controlled through the regulation of mRNA stability and translation. This post-transcriptional control is mediated by various families of RNA-binding proteins, including those with zinc finger CCCH motifs. CCCH zinc finger proteins have been shown to be essential to differentiation events in trypanosomatid parasites. Here, we functionally characterise TcZFP2 as a predicted post-transcriptional regulator of differentiation in Trypanosoma cruzi. This protein was detected in cell culture-derived amastigotes and trypomastigotes, but it was present in smaller amounts in metacyclic trypomastigote forms of T. cruzi. We use an optimised recombinant RNA immunopreciptation followed by microarray analysis assay to identify TcZFP2 target mRNAs. We further demonstrate that TcZFP2 binds an A-rich sequence in which the adenosine residue repeats are essential for high-affinity recognition. An analysis of the expression profiles of the genes encoding the TcZFP2-associated mRNAs throughout the parasite life cycle by microarray hybridisation showed that most of the associated mRNAs were upregulated in the metacyclic trypomastigote forms, also suggesting a role for TcZFP2 in metacyclic trypomastigote differentiation. Knockdown of the orthologous Trypanosoma brucei protein levels showed ZFP2 to be a positive regulator of specific target mRNA abundance. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Centre for Immunity, Infection and Evolution. School of Biological Sciences. University of Edinburgh. Edinburgh, UK. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Centre for Immunity, Infection and Evolution. School of Biological Sciences. University of Edinburgh. Edinburgh, UK. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. | pt_BR |
Subject | Zinc Fingers | pt_BR |
Subject | RNA-Binding Proteins | pt_BR |
Subject in Spanish | Dedos de Zinc | pt_BR |
Subject in Spanish | Proteínas de Unión al ARN | pt_BR |
DeCS | Dedos de Zinco | pt_BR |
DeCS | Proteínas de Ligação a RNA | pt_BR |