Author | De Simone, S. G. | |
Author | Netto, C. C. | |
Author | Silva, F. P. | |
Access date | 2020-01-16T12:29:06Z | |
Available date | 2020-01-16T12:29:06Z | |
Document date | 2006 | |
Citation | DE SIMONE, S. G. de; NETTO, C. C.; SILVA JR., F. P. Simple affinity chromatographic procedure to purify -galactoside binding lectins. Journal of Chromotherapy B, v. 838, p. 135-138, 2006. | pt_BR |
ISSN | 1570-0232 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/39250 | |
Language | eng | pt_BR |
Publisher | Elsevier | pt_BR |
Rights | restricted access | |
Subject in Portuguese | Cromatografia de afinidade | pt_BR |
Subject in Portuguese | Lectinas | pt_BR |
Subject in Portuguese | Lectinas de ligação ao galactósido do tipo C | pt_BR |
Title | Simple affinity chromatographic procedure to purify beta-galactoside binding lectins | pt_BR |
Type | Article | |
DOI | 10.1016/j.jchromb.2006.02.036 | |
Abstract | Affinity chromatography based on the commercial resin Sepharose CL-6B was used to isolate new C1-beta-type lectins from crude preparations of snake venoms (Bothrops jararaca, Bothrops jararacussu, Bothrops newiedi, Bothrops moojeni, Lachesis muta rhombeata). Most of the C-type lectins could be eluted with almost 100% recovery using the competitor isopropyl-beta-D-thiogalactoside (IPTG) or through Ca2+ sequestration with EDTA. The lectin yield varied considerably among the different snake species, but B. newiedi venom was a particularly rich source of lectin, retaining 2.7 mg of lectin by milliliter of resin in saturating conditions. C1-alpha-lectins from Crotalus durisus terrificus venom, from the jack fruit (jacalin) and from bread fruit seeds extract (frutalin) had no affinity, either with or without Ca2+ added, for Sepharose CL-6B, showing that the resin is specific for C1-beta-type lectins. Sepharose CL-6B used as galactose-affinity chromatography provides a simple and fast method for isolating C-type beta-galactoside binding lectins from crude sample preparations. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Celular. Laboratório de Bioquímica de Proteínas e Peptídeos. Rio de Janeiro, RJ, Brasil / Universidade Federal Fluminense. Instituto de Biologia. Departamento de Biologia Celular e Molecular. Niterói, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Celular. Laboratório de Bioquímica de Proteínas e Peptídeos. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Celular. Laboratório de Bioquímica de Proteínas e Peptídeos. Rio de Janeiro, RJ, Brasil. | pt_BR |
Subject | C-type -galactoside binding lectins | pt_BR |
Subject | Affinity chromatography | pt_BR |
Subject | Lectins | pt_BR |
Embargo date | 2022-01-01 | |