Author | Tornheim, Jeffrey A. | |
Author | Madugundu, Anil K. | |
Author | Paradkar, Mandar | |
Author | Fukutani, Kiyoshi Ferreira | |
Author | Queiroz, Artur Trancoso Lopo de | |
Author | Gupte, Nikhil | |
Author | Gupte, Akshay N. | |
Author | Kinikar, Aarti | |
Author | Kulkarni, Vandana | |
Author | Balasubramanian, Usha | |
Author | Sreenivasamurthy, Sreelakshmi | |
Author | Raja, Remya | |
Author | Pradhan, Neeta | |
Author | Shivakumar, Shri Vijay Bala Yogendra | |
Author | Valvi, Chhaya | |
Author | Hanna, Luke Elizabeth | |
Author | Andrade, Bruno de Bezerril | |
Author | Mave, Vidya | |
Author | Pandey, Akhilesh | |
Author | Gupta, Amita | |
Access date | 2020-01-24T11:46:28Z | |
Available date | 2020-01-24T11:46:28Z | |
Document date | 2019 | |
Citation | TORNHEIM, Jeffrey A. et al. Transcriptomic Profiles of Confirmed Pediatric Tuberculosis Patients and Household Contacts Identifies Active Tuberculosis, Infection, and Treatment Response among Indian Children. Journal of Infectious Diseases, p. 1-12, 2020. | pt_BR |
ISSN | 0022-1899 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/39488 | |
Sponsorship | US
National Institutes of Health (NIH)/Indian Department of
Biotechnology (DBT) RePORT India Consortium. This project
has been funded in whole or in part with Federal funds from
the Government of India’s DBT, the Indian Council of Medical
Research, the NIH, National Institute of Allergy and Infectious
Diseases (NIAID), Office of AIDS Research, and distributed
in part by CRDF Global. This work was also supported by the
Wellcome Trust/DBT India Alliance Margdarshi Fellowship
(Grant Number IA/M/15/1/502023; to A. P.). J. A. T. was supported
by NIH NIAID Grant K23AI135102. This work was additionally
supported by the NIH Office of the Director, Fogarty
International Center, Office of AIDS Research, National Cancer
Center, National Heart, Blood, and Lung Institute, and the NIH
Office of Research for Women’s Health through the Fogarty
Global Health Fellows Program Consortium comprised of the
University of North Carolina, John Hopkins, Morehouse, and
Tulane (R25TW009340), the Johns Hopkins University School
of Medicine Clinician Scientist Career Development Award,
and NIH NIAID (R21AI122922). Additional support came
from NIH/NIAID (UM1AI069465), the Fogarty International
Center BJGMC-JHU HIV-TB Program (D43TW009574),
the Ujala Foundation, the Gilead Foundation, the Wyncote
Foundation, and Persistent Systems. | pt_BR |
Language | eng | pt_BR |
Publisher | Oxford University Press | pt_BR |
Rights | restricted access | pt_BR |
Subject in Portuguese | Índia | pt_BR |
Subject in Portuguese | TB pediátrica | pt_BR |
Subject in Portuguese | Diagnóstico de TB | pt_BR |
Subject in Portuguese | Transcriptômica | pt_BR |
Title | Transcriptomic Profiles of Confirmed Pediatric Tuberculosis Patients and Household Contacts Identifies Active Tuberculosis, Infection, and Treatment Response among Indian Children | pt_BR |
Type | Article | pt_BR |
DOI | 10.1093/infdis/jiz639 | |
Abstract | Gene expression profiling is emerging as a tool for tuberculosis diagnosis and treatment response monitoring, but limited data specific to Indian children and incident tuberculosis infection (TBI) exist. Methods. Sixteen pediatric Indian tuberculosis cases were age- and sex-matched to 32 tuberculosis-exposed controls (13 developed
incident TBI without subsequent active tuberculosis). Longitudinal samples were collected for ribonucleic acid sequencing.
Differential expression analysis generated gene lists that identify tuberculosis diagnosis and tuberculosis treatment response. Data
were compared with published gene lists. Population-specific risk score thresholds were calculated.
Results. Seventy-one genes identified tuberculosis diagnosis and 25 treatment response. Within-group expression was partially
explained by age, sex, and incident TBI. Transient changes in gene expression were identified after both infection and treatment.
Application of 27 published gene lists to our data found variable performance for tuberculosis diagnosis (sensitivity 0.38–1.00,
specificity 0.48–0.93) and treatment response (sensitivity 0.70–0.80, specificity 0.40–0.80). Our gene lists found similarly variable
performance when applied to published datasets for diagnosis (sensitivity 0.56–0.85, specificity 0.50–0.85) and treatment response
(sensitivity 0.49– 0.86, specificity 0.50–0.84).
Conclusions. Gene expression profiles among Indian children with confirmed tuberculosis were distinct from adult-derived
gene lists, highlighting the importance of including distinct populations in differential gene expression models. | pt_BR |
Affilliation | Johns Hopkins University. School of Medicine. Center for Clinical Global Health Education. Division of Infectious Diseases. Baltimore, Maryland, USA. | pt_BR |
Affilliation | Institute of Bioinformatics. Bangalore, Karnataka, India / National Institute of Mental Health and Neurosciences Center for Molecular Medicine. Bangalore, India / Manipal Academy of Higher Education. Manipal, Karnataka, India / Mayo Clinic. Center for Individualized Medicine. Department of Laboratory Medicine and Pathology Rochester. Minnesota, USA. | pt_BR |
Affilliation | Johns Hopkins University Clinical Research Site. Byramjee Jeejeebhoy Government Medical College. Pune, Maharashtra, India. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Multinational Organization Network Sponsoring Translational and Epidemiological Research Initiative. Salvador, BA, Brazil / Faculdade de Tecnologia e Ciências. Salvador, BA, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. | pt_BR |
Affilliation | Johns Hopkins University. School of Medicine. Center for Clinical Global Health Education. Division of Infectious Diseases. Baltimore, Maryland, USA / Johns Hopkins University Clinical Research Site. Byramjee Jeejeebhoy Government Medical College. Pune, Maharashtra, India. | pt_BR |
Affilliation | Johns Hopkins University. School of Medicine. Center for Clinical Global Health Education. Division of Infectious Diseases. Baltimore, Maryland, USA | pt_BR |
Affilliation | Byramjee Jeejeebhoy Government Medical College. Pune, Maharashtra, India. | pt_BR |
Affilliation | Johns Hopkins University Clinical Research Site. Byramjee Jeejeebhoy Government Medical College. Pune, Maharashtra, India. | pt_BR |
Affilliation | Johns Hopkins University Clinical Research Site. Byramjee Jeejeebhoy Government Medical College. Pune, Maharashtra, India. | pt_BR |
Affilliation | Institute of Bioinformatics. Bangalore, Karnataka, India / National Institute of Mental Health and Neurosciences Center for Molecular Medicine. Bangalore, India / Johns Hopkins University School of Medicine. McKusick-Nathans Institute of Genetic Medicine. Baltimore, Maryland, USA. | pt_BR |
Affilliation | Institute of Bioinformatics. Bangalore, Karnataka, India / National Institute of Mental Health and Neurosciences Center for Molecular Medicine. Bangalore, India / Manipal Academy of Higher Education. Manipal, Karnataka, India. | pt_BR |
Affilliation | Johns Hopkins University Clinical Research Site. Byramjee Jeejeebhoy Government Medical College. Pune, Maharashtra, India. | pt_BR |
Affilliation | Johns Hopkins University. India office Center for Clinical Global Health Education. Pune, Maharashtra, India. | pt_BR |
Affilliation | Jeejeebhoy Government Medical College. Pune, Maharashtra, India. | pt_BR |
Affilliation | National Institute for Research in Tuberculosis. Chennai, Tamil Nadu, India. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Multinational Organization Network Sponsoring Translational and Epidemiological Research Initiative. Salvador, BA, Brazil / Faculdade de Tecnologia e Ciências. Salvador, BA, Brasil / Universidade Salvador. Laureate Universities, Salvador, BA, Brasil / Escola Bahiana de Medicina e Saúde Pública. Salvador, BA, Brasil. | pt_BR |
Affilliation | Johns Hopkins University. School of Medicine. Center for Clinical Global Health Education. Division of Infectious Diseases. Baltimore, Maryland, USA / Johns Hopkins University Clinical Research Site. Byramjee Jeejeebhoy Government Medical College. Pune, Maharashtra, India. | pt_BR |
Affilliation | Institute of Bioinformatics. Bangalore, Karnataka, India / National Institute of Mental Health and Neurosciences Center for Molecular Medicine. Bangalore, India / Mayo Clinic. Center for Individualized Medicine. Department of Laboratory Medicine and Pathology Rochester. Minnesota, USA / Johns Hopkins University School of Medicine. McKusick-Nathans Institute of Genetic Medicine, , Baltimore, Maryland, USA. | pt_BR |
Affilliation | Johns Hopkins University. School of Medicine. Center for Clinical Global Health Education. Division of Infectious Diseases. Baltimore, Maryland, USA / Johns Hopkins Bloomberg School of Public Health. Baltimore, Maryland, USA. | pt_BR |
Subject | India | pt_BR |
Subject | Pediatric TB | pt_BR |
Subject | TB diagnosis | pt_BR |
Subject | Transcriptomics | pt_BR |