Author | Silva, Gabriela O. Palva | |
Author | Oliveira, Christine Cruz | |
Author | Nakayasu, Ernesto S. | |
Author | Maya-Monteiro, Clarissa M. | |
Author | Dunkov, Boris C. | |
Author | Masuda, Hatisaburo | |
Author | Almeida, Igor C. | |
Author | Oliveira, Pedro L. | |
Access date | 2020-01-29T15:56:42Z | |
Available date | 2020-01-29T15:56:42Z | |
Document date | 2006 | |
Citation | SILVA, Gabriela O. Paiva et al. A heme-degradation pathway in a blood-sucking insect. PNAS, v. 103, n. 21, p. 8030-8035, May 2006. | pt_BR |
ISSN | 0027-8424 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/39586 | |
Language | eng | pt_BR |
Publisher | National Academy of Sciences | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | Rhodnius | pt_BR |
Subject in Portuguese | Estresse oxidativo | pt_BR |
Subject in Portuguese | Biliverdin | pt_BR |
Subject in Portuguese | Heme oxygenase | pt_BR |
Title | A heme-degradation pathway in a blood-sucking insect | pt_BR |
Type | Article | pt_BR |
DOI | 10.1073/pnas.0602224103 | |
Abstract | Hematophagous insects are vectors of diseases that affect hundreds of millions of people worldwide. A common physiological event in the life of these insects is the hydrolysis of host hemoglobin in the digestive tract, leading to a massive release of heme, aknownprooxidantmolecule.Diverseorganisms,frombacteriato plants, express the enzyme heme oxygenase, which catalyzes the oxidative degradation of heme to biliverdin (BV) IX, CO, and iron. Here, we show that the kissing bug Rhodnius prolixus, a vector of Chagas’disease,hasauniqueheme-degradationpathwaywherein heme is first modified by addition of two cysteinylglycine residues beforecleavageoftheporphyrinring,followedbytrimmingofthe dipeptides. Furthermore, in contrast to most known heme oxygenases, which generate BV IX , in this insect, the end product of hemedetoxificationisadicysteinyl-BVIX .Basedontheseresults, we propose a heme metabolizing pathway that includes the identified intermediates produced during modification and cleavage of the heme porphyrin ring. | pt_BR |
Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | University of Texas. Department of Biological Sciences. El Paso, TX, USA / Universidade de São Paulo. Departamento de Parasitologia. São Paulo, SP, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Fisiologia e Farmacodinâmica. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | University of Arizona. Center for Insect Science. Department of Biochemistry and Molecular Biophysics. Tucson, AZ, USA. | pt_BR |
Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | University of Texas. Department of Biological Sciences. El Paso, TX, USA / Universidade de São Paulo. Departamento de Parasitologia. São Paulo, SP, Brasil. | pt_BR |
Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis. Rio de Janeiro, RJ, Brasil / | pt_BR |
Subject | Biliverdin | pt_BR |
Subject | Heme oxygenase | pt_BR |
Subject | Oxidative stress | pt_BR |
Subject | Rhodnius | pt_BR |
e-ISSN | 1091-6490 | |