Author | Michel, Marie-Laure | |
Author | Cruz, Daniella Mendes da | |
Author | Keller, Alexandre Castro | |
Author | Lochner, Matthias | |
Author | Schneider, Elke | |
Author | Dy, Michel | |
Author | Ebert, Gérard | |
Author | Moraes, Maria C. Leite de | |
Access date | 2020-02-11T14:08:55Z | |
Available date | 2020-02-11T14:08:55Z | |
Document date | 2008 | |
Citation | MICHEL, Marie-Laure et al. Critical role of ROR t in a new thymic pathway leading to IL-17-producing invariant NKT cell differentiation. PNAS, v; 105. n. 50, p. 19845-19850, Dec. 2008. | pt_BR |
ISSN | 0027-8424 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/39842 | |
Language | eng | pt_BR |
Publisher | National Academy of Sciences | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | Células T Matadoras Naturais | pt_BR |
Subject in Portuguese | Citocinas | pt_BR |
Title | Critical role of ROR t in a new thymic pathway leading to IL-17-producing invariant NKT cell differentiation | pt_BR |
Type | Article | pt_BR |
DOI | 10.1111/j.1472-765X.2008.02322.x | |
Abstract | Invariant natural killer T (iNKT) cells constitute a subpopulation of T
cells that recognize glycolipids presented by CD1d molecules. They
are characterized by their prompt production of interleukin-4 (IL-4)
and interferon- (IFN- ), which enables them to modulate diverse
immune responses. Recently, we enlarged this concept by identifying
a distinct IL-17-producing iNKT cell subset, named iNKT17 cells. The
mechanisms leading to the acquisition of this new iNKT cell activity
are unknown. Herein we show that IL-17-producing iNKT cells are
already present in the thymus, predominantly among a subset regarded so far as an immature stage of thymic iNKT cell development,
the CD1d tetramerposCD44posNK1.1negCD4neg cells. Using EGFP reporter mice, we demonstrate that the transcription factor ROR- t is
critical for the thymic differentiation of this subset because only
ROR- tpos iNKT cells are capable of massively secreting IL-17. Moreover, IL-17-producing CD1d tetramerposCD44posNK1.1negCD4neg thymic iNKT cells have reached a mature differentiation stage because
they fail to generate other cell subsets in fetal thymic organ culture.
Conversely, thymic ROR- tneg iNKT cell precursors give rise to progeny, but acquire neither ROR- t expression nor the ability to secrete
IL-17. In conclusion, our findings demonstrate an alternative thymic
pathway leading to the development of iNKT17 cells that requires
ROR- t expression. | pt_BR |
Affilliation | Université Paris Descartes. Faculté de Médecine. Centre National de la Recherche Scientifique. Unité Mixte de Recherche 8147. Hôpital Necker. Paris, France. | pt_BR |
Affilliation | Université Paris Descartes. Faculté de Médecine. Centre National de la Recherche Scientifique. Unité Mixte de Recherche 8147. Hôpital Necker. Paris, France / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisas sobre o Timo. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Université Paris Descartes. Faculté de Médecine. Centre National de la Recherche Scientifique. Unité Mixte de Recherche 8147. Hôpital Necker. Paris, France / University Federal de Sao Paulo. São Paulo, SP, Brasil. | pt_BR |
Affilliation | Institut Pasteur. Centre National de la Recherche Scientifique. Unité de Recherche Associée 1961. Laboratory of Lymphoid Tissue Development. Paris, France. | pt_BR |
Affilliation | Université Paris Descartes. Faculté de Médecine. Centre National de la Recherche Scientifique. Unité Mixte de Recherche 8147. Hôpital Necker. Paris, France. | pt_BR |
Affilliation | Université Paris Descartes. Faculté de Médecine. Centre National de la Recherche Scientifique. Unité Mixte de Recherche 8147. Hôpital Necker. Paris, France. | pt_BR |
Affilliation | Institut Pasteur. Centre National de la Recherche Scientifique. Unité de Recherche Associée 1961. Laboratory of Lymphoid Tissue Development. Paris, France. | pt_BR |
Affilliation | Université Paris Descartes. Faculté de Médecine. Centre National de la Recherche Scientifique. Unité Mixte de Recherche 8147. Hôpital Necker. Paris, France. | pt_BR |
Subject | Thymic precursors | pt_BR |
Subject | -GalCer | pt_BR |
Subject | CD1d | pt_BR |
Subject | Cytokines | pt_BR |
e-ISSN | 1091-6490 | |