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EXTERNAL CONTROL VIRAL-LIKE PARTICLE CONSTRUCTION FOR DETECTION OF EMERGENT ARBOVIRUSES BY REAL-TIME REVERSE-TRANSCRIPTION PCR
Reverse Transcriptase Polymerase Chain Reaction
Reagent Kits, Diagnostic
Juego de Reactivos para Diagnóstico
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Kit de Reagentes para Diagnóstico
Autor(es)
Afiliação
Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil / Universidade Federal do Paraná. Programa de Pós-Graduação em Engenharia de Bioprocessos e Biotecnologia. Curitiba, PR, Brasil.
Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil.
Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil.
Fundação Oswaldo Cruz. Escritório Rondônia. Porto Velho, Brasil.
Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil / Universidade Federal do Paraná. Programa de Pós-Graduação em Engenharia de Bioprocessos e Biotecnologia. Curitiba, PR, Brasil / Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.
Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil.
Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil.
Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil.
Fundação Oswaldo Cruz. Escritório Rondônia. Porto Velho, Brasil.
Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil / Universidade Federal do Paraná. Programa de Pós-Graduação em Engenharia de Bioprocessos e Biotecnologia. Curitiba, PR, Brasil / Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.
Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil.
Resumo em Inglês
Arboviruses have been emerging and reemerging worldwide, predominantly in tropical and subtropical areas. As many arbovirus infections, including dengue (DENV), Zika (ZIKV), and chikungunya (CHIKV), have similar signs and symptoms, clinical diagnosis of arbovirus infections is challenging. Therefore, reliable laboratory tests are necessary to improve the clinical management of patients with suspected arbovirus infections. Real-time reverse-transcription PCR (RT-qPCR) is among the more effective methods to distinguish these viruses. The aim of this study was to construct a unique positive external control derived from a unique plasmid using genetic engineering for specific use in RT-qPCR assays to detect Zika, dengue (1-4), and chikungunya. An external control derived from the MS2 bacteriophage was constructed using sequences from arbovirus and human genomes. Laboratories were asked to test the control in the ZDC Biomol kit, a RT-qPCR kit which is able to detect Zika, dengue serotypes 1-4, chikungunya, and an internal human control. RNA extracted from the external control was able to be amplified and detected in RT-qPCR assays for each virus detected by using the ZDC Biomol kit. The external control, samples from viral culture, and infected patient samples display similar amplification using this assay. The pET47b(+)MS2-ZDC vector is a viable expression system for the production of external control viral-like particles (MS2-ZDC). The RNA from the recombinant particles can be easily extracted and can function as a tool to validate all steps of process from the extraction to the amplification of all targets in specific reaction. Thus, the MS2-ZDC particles are laboratory-safe in order to avoid risk for operators, and the phages are effective as positive control for use in the ZDC Biomol kit amplifying all kit targets making them effective for commercial profile.
Palavras-chave
PCRPalavras-chave em inglês
ArbovirusesReverse Transcriptase Polymerase Chain Reaction
Reagent Kits, Diagnostic
Palavras-chave em espanhol
Reacción en Cadena de la Polimerasa de Transcriptasa InversaJuego de Reactivos para Diagnóstico
DeCS
ArbovirusReação em Cadeia da Polimerase Via Transcriptase Reversa
Kit de Reagentes para Diagnóstico
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