| Author | Souza, Elen Mello de | |
| Author | Henriques-Pons, Andrea | |
| Author | Bailly, Christian | |
| Author | Lansiaux, Amelie | |
| Author | Araujo-Jorge, Tania C. | |
| Author | Soeiro, Maria de Nazaré C. | |
| Access date | 2020-03-24T17:43:19Z | |
| Available date | 2020-03-24T17:43:19Z | |
| Document date | 2004 | |
| Citation | SOUZA, Elen Mello de et al. In vitro measurement of enzymatic markers as a tool to detect mouse cardiomyocytes injury. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 99, n. 7, p. 697-701, Nov. 2004. | pt_BR |
| ISSN | 0074-0276 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/40487 | |
| Language | eng | pt_BR |
| Publisher | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. | pt_BR |
| Rights | open access | pt_BR |
| Subject in Portuguese | Creatina quinase | pt_BR |
| Subject in Portuguese | Ensaios in vitro | pt_BR |
| Subject in Portuguese | Danos cardíacos | pt_BR |
| Title | In vitro measurement of enzymatic markers as a tool to detect mouse cardiomyocytes injury | pt_BR |
| Type | Article | pt_BR |
| DOI | 10.1590/s0074-02762004000700006 | |
| Abstract | Primary cultures of cardiomyocytes represent a useful model for analyzing cardiac cell biology as well as pathogenesis of several cardiovascular disorders. Our aim was to standardize protocols for determining the damage of cardiac cells cultured in vitro by measuring the creatine kinase and its cardiac isotype and lactate dehydrogenase activities in the supernatants of mice cardiomyocytes submitted to different protocols of cell lysis. Our data showed that due to its higher specificity, the cardiac isotype creatine kinase was the most sensitive as compared to the others studied enzymatic markers, and can be used to monitor and evaluate cardiac damage in in vitro assays. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Ultra-estrutura e Biologia Celular. Laboratório de Biologia Celular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Ultra-estrutura e Biologia Celular. Laboratório de Biologia Celular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Institute de Recherches sur le Cancer de Lille. Centre Oscar Lambret. INSERM U-524/Laboratoire de Pharmacologie Antitumorale. Lille, France. | pt_BR |
| Affilliation | Institute de Recherches sur le Cancer de Lille. Centre Oscar Lambret. INSERM U-524/Laboratoire de Pharmacologie Antitumorale. Lille, France. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Ultra-estrutura e Biologia Celular. Laboratório de Biologia Celular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Ultra-estrutura e Biologia Celular. Laboratório de Biologia Celular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Creatine kinase | pt_BR |
| Subject | In vitro assays | pt_BR |
| Subject | Cardiac damage | pt_BR |
| e-ISSN | 1678-8060 | |
