| Author | Almeida, Isabela Neves de | |
| Author | Vasconcellos, Sidra Ezidio Gonçalves | |
| Author | Figueredo, Lida Jouca de Assis | |
| Author | Dantas, Nayanne Gama Teixeira | |
| Author | Augusto, Cláudio José | |
| Author | Hadaad, João Paulo Amaral | |
| Author | Suffys, Philip Noel | |
| Author | Carvalho, Wânia da Silva | |
| Author | Miranda, Silvana Spíndola de | |
| Access date | 2020-03-28T17:10:51Z | |
| Available date | 2020-03-28T17:10:51Z | |
| Document date | 2019 | |
| Citation | ALMEIDA, Isabela Neves et al. Frequency of the Mycobacterium tuberculosis RDRio genotype and its association with multidrug-resistant tuberculosis. BMC Infectious Diseases, v. 18, n. 556, p. 1-9, 2019. | pt_BR |
| ISSN | 1471-2334 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/40548 | |
| Language | eng | pt_BR |
| Publisher | BMC | pt_BR |
| Rights | open access | pt_BR |
| Subject in Portuguese | Tuberculose | pt_BR |
| Subject in Portuguese | Mycobacterium tuberculosis | pt_BR |
| Subject in Portuguese | Genótipo | pt_BR |
| Subject in Portuguese | Tuberculose Resistente a Múltiplos Medicamentos | pt_BR |
| Subject in Portuguese | Nucleotídeo único | pt_BR |
| Title | Frequency of the Mycobacterium tuberculosis RDRio genotype and its association with multidrug-resistant tuberculosis | pt_BR |
| Type | Article | pt_BR |
| DOI | 10.1186/s12879-019-4152-7 | |
| Abstract | Background: In recent decades, Mycobacterium tuberculosis with the RDRio genotype, frequently isolated from tuberculosis patients in Rio de Janeiro, has become part of the Latin American – Mediterranean (LAM) family and has been associated with multidrug-resistant tuberculosis (MDR-TB). The aim of this study was to investigate the frequency of M. tuberculosis RDRio in the state of Minas Gerais, Brazil, and its relationship with MDR-TB. Methods: For convenience, 172 susceptible and 63 MDR M. tuberculosis isolates were taken from pulmonary samples from patients diagnosed between January 2007 and December 2011. The DNA extracted from these isolates was analyzed by spoligotyping, PCR-RFLP to characterize fbpC103/Ag85C103, multiplex PCR to detect RDRio and RD174, and MIRU-VNTR 24 loci. Results: Among the 235 isolates, the RDRio pattern was identified in 122 (51.9%) isolates (IC 0.45–0.58), with 100 (42.5%) wild-type and 13 (5.5%) mixed pattern isolates, whereas RD174 was identified in 93 of the 122 RDRio positive samples (76.3%). The LAM family and the LAM9 lineage were the most frequently identified among the isolates in this study. Among the 63 MDR isolates, 41 (65.1%) were RDRio and 28 (44.4%) RD174. Conclusion: The association of both deletions with MDR proved to be statistically significant, corroborating the few reports that have associated RDRio with MDR. | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Faculdade de Medicina. Laboratório de Pesquisa em Micobactérias. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular Aplicada a Micobactérias. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Faculdade de Medicina. Laboratório de Pesquisa em Micobactérias. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Faculdade de Medicina. Laboratório de Pesquisa em Micobactérias. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Fundação Ezequiel Dias. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Escola de Veterinária. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular Aplicada a Micobactérias. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Faculdade de Farmácia. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Faculdade de Medicina. Laboratório de Pesquisa em Micobactérias. Belo Horizonte, MG, Brasil. | pt_BR |
| Subject | Tuberculosis | pt_BR |
| Subject | Mycobacterium tuberculosis | pt_BR |
| Subject | Multidrug-resistant tuberculosis | pt_BR |
| Subject | Genotype | pt_BR |
| Subject | Single nucleotide | pt_BR |
| xmlui.metadata.dc.subject.ods | 03 Saúde e Bem-Estar | |
