Author | Ferreira, Jéssica da Silva | |
Author | Carvalho, Fernanda Marques de | |
Author | Pessolani, Maria Cristina Vidal | |
Author | Antunes, João Marcelo Azevedo de Paula | |
Author | Oliveira, Ilanna Vanessa Pristo de Medeiros | |
Author | Moura, Gabriela Hémylin Ferreira | |
Author | Truman, Richard Wayne | |
Author | Peña, Maria Teresa | |
Author | Sharma, Rahul | |
Author | Duthie, Malcolm S. | |
Author | Guimarães, Ricardo José de Paula Souza e | |
Author | Fontes, Amanda Nogueira Brum | |
Author | Suffys, Philip N. | |
Author | McIntosh, Douglas | |
Access date | 2020-04-27T18:28:04Z | |
Available date | 2020-04-27T18:28:04Z | |
Document date | 2020 | |
Citation | FERREIRA, Jéssica da Silva et al. Serological and molecular detection of infection with Mycobacterium leprae in Brazilian six banded armadillos (Euphractus sexcinctus). Comparative Immunology, Microbiology and Infectious Diseases, v. 68, p. 1-8, 2020. | pt_BR |
ISSN | 0147-9571 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/40996 | |
Description | Acesso aberto em 26/04/2020 - https://www.sciencedirect.com/science/article/pii/S0147957119301948?via%3Dihub | pt_BR |
Language | eng | pt_BR |
Publisher | Elsevier 12 Months | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | Tatu | pt_BR |
Subject in Portuguese | Imunodiagnóstico | pt_BR |
Subject in Portuguese | Hanseníase | pt_BR |
Subject in Portuguese | Zoonóticos | pt_BR |
Subject in Portuguese | PCR | pt_BR |
Subject in Portuguese | Reação em Cadeia da Polimerase | pt_BR |
Title | Serological and molecular detection of infection with Mycobacterium leprae in Brazilian six banded armadillos (Euphractus sexcinctus) | pt_BR |
Type | Article | pt_BR |
DOI | 10.1016/j.cimid.2019.101397 | |
Abstract | Leprosy was recognized as a zoonotic disease, associated with nine-banded armadillos (Dasypus novemcinctus) in the Southern United States of America in 2011. In addition, there is growing evidence to support a role for armadillos in zoonotic leprosy in South America. The current study evaluated twenty specimens of the six-banded armadillo (Euphractus sexcinctus), collected from rural locations in the state of Rio Grande do Norte (RN), Brazil for evidence of infection with Mycobacterium leprae. Serum was examined using two "in-house" enzyme-linked immunosorbent assays (ELISAs) and via two commercially available (ML flow and NDO-LID®) immunochromatographic lateral flow (LF) tests, for detection of the PGL-I and/or LID-1 antigens of the bacterium. The presence of M. leprae DNA in liver tissue was examined using the multi-copy, M. leprae-specific repetitive element (RLEP), as target in conventional and nested PCR assays. Molecular and anti-PGL-I-ELISA data indicated that 20/20 (100 %) of the armadillos were infected with M. leprae. The corresponding detection levels recorded with the LF tests were 17/20 (85 %) and 16/20 (85 %), for the NDO-LID® and ML flow tests, respectively. Our results indicate that, in common with D. novemcinctus, six banded armadillos (a species hunted and reared as a food-source in some regions of Brazil, including RN), represent a potential reservoir of M. leprae and as such, their role in a possible zoonotic cycle of leprosy within Brazil warrants further investigation. | pt_BR |
Affilliation | Universidade Federal Rural do Rio de Janeiro. Instituto de Medicina Veterinária. Departamento de Parasitologia Animal. Laboratório de Biologia Molecular Multiusuários. Seropédica, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Universidade Federal Rural Sem-Árido. Hospital Veterinário. RN, Brasil. | pt_BR |
Affilliation | Universidade Federal Rural Sem-Árido. Hospital Veterinário. RN, Brasil. | pt_BR |
Affilliation | Universidade Federal Rural Sem-Árido. Hospital Veterinário. RN, Brasil. | pt_BR |
Affilliation | National Hansen's Disease Program. Health Resources and Services Administration, Healthcare Systems Bureau. Department of Health and Humans Services. Baton Rouge, USA. | pt_BR |
Affilliation | National Hansen's Disease Program. Health Resources and Services Administration, Healthcare Systems Bureau. Department of Health and Humans Services. Baton Rouge, USA. | pt_BR |
Affilliation | National Hansen's Disease Program. Health Resources and Services Administration, Healthcare Systems Bureau. Department of Health and Humans Services. Baton Rouge, USA. | pt_BR |
Affilliation | Infectious Disease Research Institut. Seattle, USA. | pt_BR |
Affilliation | Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular Aplicada a Micobactérias. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular Aplicada a Micobactérias. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Universidade Federal Rural do Rio de Janeiro. Instituto de Medicina Veterinária. Departamento de Parasitologia Animal. Laboratório de Biologia Molecular Multiusuários. Seropédica, RJ, Brasil. | pt_BR |
Subject | Armadillo | pt_BR |
Subject | Immunodiagnostics | pt_BR |
Subject | Leprosy | pt_BR |
Subject | PCR | pt_BR |
Subject | Zoonotic | pt_BR |
e-ISSN | 1878-1667 | |