Author | Silva, Simone da | |
Author | Alencar, Danielle Cardoso | |
Author | Benevides, Paulo José Coelho | |
Author | Astolfi-Filho, Spartaco | |
Access date | 2020-05-18T23:19:46Z | |
Available date | 2020-05-18T23:19:46Z | |
Document date | 2020 | |
Citation | SILVA, Simone da et al. Emetine and cephaeline production and regulation by in vitro propagation of Psychotria ipecacuanha (Brot.) Stokes in semi-solid media and temporary immersion bioreactor. Revista Fitos, Rio de Janeiro, v. 14, n. 1, p. 45-55, 2020. | en_US |
ISSN | 1808-9569 | en_US |
URI | https://www.arca.fiocruz.br/handle/icict/41292 | |
Abstract in Portuguese | Psychotria ipecacuanha, is a plant species with known medicinal properties that is critically endangered due to overexploitation of natural populations. Although the difficulties in conventional propagation by seed and by vegetative propagation are generally understood, the present study enhances our knowledge by describing efficient plant regeneration and root induction protocols for P. ipecacuanha while comparing alkaloid content (emetine and cephaeline) in in vitro-derived tissues. Stem node explants were cultured on MS medium MS supplemented with indolbutiric acid (IBA) in semi-solid media and the RITA® temporary immersion bioreactor. The highest root formation (81%) was in MS + 1.5 mg L−1 IBA in the bioreactor. After 24 months of acclimatization, the plants cultivated in MS + 0.50 and 1.0 mg L−1 of IBA had the highest number of roots (3), with mean values of 10.47 and 9.40 cm, respectively. The cultures coming from 1.0 mg L−1 and 0.5 mg L−1 IBA in the bioreactor contained higher cephaeline content, with a relative area of 14.2 and 14.9%, respectively. For emetine, the 1.0 mg L−1 IBA cultures in the bioreactor, 0.5 mg L−1 IBA and MS0 cultures contained higher content than the other treatments, with a relative area of 10.2, 10.2 and 10.1%, respectively. | en_US |
Language | eng | en_US |
Publisher | Fundação Oswaldo Cruz. Farmanguinhos. Núcleo de Gestão em Biodiversidade e Saúde | en_US |
Rights | open access | |
Subject in Portuguese | Plant Biotechnology | en_US |
Subject in Portuguese | Tissue Culture | en_US |
Subject in Portuguese | Ipecac | en_US |
Subject in Portuguese | Temporary Immersion | en_US |
Subject in Portuguese | Secondary Metabolites | en_US |
Title | Emetine and cephaeline production and regulation by in vitro propagation of Psychotria ipecacuanha (Brot.) Stokes in semi-solid media and temporary immersion bioreactor | en_US |
Type | Article | en_US |
DOI | 10.32712/2446-4775.2020.799 | |
Affilliation | Centro de Biotecnologia da Amazônia. Coordenação de Biotecnologia Vegetal. Manaus, AM, Brasil. | pt_BR |
Affilliation | Centro de Biotecnologia da Amazônia. Coordenação de Química e Farmacognosia. Manaus, AM, Brasil. | pt_BR |
Affilliation | Centro de Biotecnologia da Amazônia. Coordenação de Química e Farmacognosia. Manaus, AM, Brasil. | pt_BR |
Affilliation | Universidade Federal do Amazonas. Instituto de Ciências Biológicas. Departamento de Genética. Manaus, AM, Brasil. | pt_BR |
e-ISSN | 2446-4775 | |