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2025-12-31
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CD4 AND CD8 DISTRIBUTION PROFILE IN INDIVIDUALS INFECTED BY SCHISTOSOMA MANSONI
CD28 antigens
Mitogen-Activated Protein Kinase
Schistosoma mansoni
Antígenos CD28
Linfócitos T CD4-Positivos
Linfócitos T CD8-Positivos
Citometria de Fluxo
Antígenos HLA-DR
Proteína Quinase 1 Ativada por Mitógeno
Proteína Quinase 3 Ativada por Mitógeno
Schistosoma mansoni
Autor
Afiliación
Universidade Federal de Minas Gerais. Departamento de Patologia. Belo Horizonte, MG, Brasil.
Fundação Oswaldo Cruz. Fiocruz Brasília. Brasília, DF, Brasil.
Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brasil.
Universidade Federal de Minas Gerais. Faculdade de Medicina. Departamento de Medicina Interna. Belo Horizonte, MG, Brasil.
Universidade Federal de Minas Gerais. Escola de Enfermagem. Belo Horizonte, MG, Brasil.
Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brasil.
Universidade Federal de Minas Gerais. Departamento de Cirurgia. Belo Horizonte, MG, Brasil.
Fundação Oswaldo Cruz. Fiocruz Brasília. Brasília, DF, Brasil.
Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brasil.
Universidade Federal de Minas Gerais. Faculdade de Medicina. Departamento de Medicina Interna. Belo Horizonte, MG, Brasil.
Universidade Federal de Minas Gerais. Escola de Enfermagem. Belo Horizonte, MG, Brasil.
Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brasil.
Universidade Federal de Minas Gerais. Departamento de Cirurgia. Belo Horizonte, MG, Brasil.
Resumen en ingles
Rationale: Patients with chronic Schistosoma mansoni infection show lower antisoluble egg antigen (SEA) proliferation responses and higher responses to soluble worm antigen preparation (SWAP). Objective: To compare the activation status and proliferation response of peripheral blood mononuclear cells (PBMC) of infected (XTO) and egg-negative individuals (NI) living in the same endemic area. Methods: XTO (n = 51) and NI individuals from the same geographical area (n = 37) and healthy blood donors (n = 22) were evaluated before and after stimulation with SEA and SWAP. The expression of activation markers (CD4+ HLADR+, CD8high+HLA-DR+ and CD8+ CD28+) and proliferation assay was assessed by flow cytometry. Findings: PBMC from infected patients showed lower frequency of CD4+ but no change in CD8+ T cells when compared with the healthy donor group. The ratio CD4+ ⁄CD8+ was 1.3, 0.6 and 0.5 in healthy donors, infected and non-infected individuals, respectively. The HLA-DR+ expression on CD8+ was higher in PBMC from infected and noninfected individuals than from healthy donors, but similar in both total lymphocytes and CD4+ populations. No intergroup proliferation response differences were observed in CD4+ and CD8+ PBMC unstimulated and stimulated with SEA and SWAP. The SEA but not SWAP-stimulated cells showed a decrease in the expression of phosphorylated extracellular signal-regulated kinase (ERK1⁄ 2). Conclusions: XTO and NI individuals living in the same area presented a smaller per cent of CD4+ and a higher per cent of CD8+ cells. The activation by either CD8high+HLA-DR+ or CD8high+HLA-DR+ ⁄CD8+ was enhanced and decreased in XTO and NI by CD8+ CD28+ and CD8+ CD28+ ⁄CD8+ when compared with healthy donor. ERK phosphorylation was attenuated in XTO and NI individuals when stimulated with SEA but not SWAP.
Palabras clave en ingles
HLA-DR antigensCD28 antigens
Mitogen-Activated Protein Kinase
Schistosoma mansoni
DeCS
Antígenos de HelmintosAntígenos CD28
Linfócitos T CD4-Positivos
Linfócitos T CD8-Positivos
Citometria de Fluxo
Antígenos HLA-DR
Proteína Quinase 1 Ativada por Mitógeno
Proteína Quinase 3 Ativada por Mitógeno
Schistosoma mansoni
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