Autor | Rodrigues, Bruno Leite | |
Autor | Baton, Luke Antony | |
Autor | Shimabukuro, Paloma Helena Fernandes | |
Data de acesso | 2020-07-21T15:24:02Z | |
Data de disponibilização | 2020-07-21T15:24:02Z | |
Data do publicação | 2020 | |
Citação | RODRIGUES, Bruno Leite; BATON, Luke Antony; SHIMABUKURO, Paloma Helena Fernandes Single-locus DNA barcoding and species delimitation of the sandfly subgenus Evandromyia(Aldamyia). Medical And Veterinary Entomology, 2020. doi.org/10.1111/mve.12458 | pt_BR |
ISSN | 1365-2915 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/42325 | |
Idioma | eng | pt_BR |
Editor | Wiley Online Library | pt_BR |
Direito Autoral | restricted access | pt_BR |
Título | Single-locus DNA barcoding and species delimitation of the sandfly subgenus Evandromyia (Aldamyia) | pt_BR |
Tipo do documento | Article | pt_BR |
DOI | https://doi.org/10.1111/mve.12458 | |
Resumo em Inglês | Sandfly specimens from the subgenus Evandromyia (Aldamyia ) Galati, 2003 (Diptera: Psychodidae: Phlebotominae) were collected between 2012 and 2019 from nine localities in seven Brazilian states, morphologically‐identified, and then DNA barcoded by sequencing the mitochondrial cytochrome c oxidase subunit I (coi ) gene. Forty‐four new barcode sequences generated from 10 morphospecies were combined with 49 previously published sequences from the same subgenus and analysed using sequence‐similarity methods (best‐match criteria) to assess their ability at specimen identification, while four different species delimitation methods (ABGD, GMYC, PTP and TCS) were used to infer molecular operational taxonomic units (MOTUs). Overall, seven of the 11 morphospecies analysed were congruent with both the well‐supported clades identified by phylogenetic analysis and the MOTUs inferred by species delimitation, while the remaining four morphospecies – E. carmelinoi , E. evandroi , E. lenti and E. piperiformis – were merged into a single well‐supported clade/MOTU. Although E. carmelinoi , E. evandroi and E. lenti were indistinguishable using coi DNA barcodes, E. piperiformis did form a distinct phylogenetic cluster and could be correctly identified using best‐match criteria. Despite their apparent morphological differences, we propose on the basis of the molecular similarity of their DNA barcodes that these latter four morphospecies should be considered members of a recently‐diverged species complex. | pt_BR |
Afiliação | Fundação Oswaldo Cruz. René Rachou. Grupo de Estudos em Leishmanioses. Belo Horizonte, MG, Brazil. | pt_BR |
Afiliação | 50 Rowntree Way Saffron Walden, Essex, CB11 4DL, U.K. | pt_BR |
Afiliação | Fundação Oswaldo Cruz. René Rachou. Grupo de Estudos em Leishmanioses. Belo Horizonte, MG, Brazil. | pt_BR |
Palavras-chave em inglês | coi | pt_BR |
Palavras-chave em inglês | Evandromyia | pt_BR |
Palavras-chave em inglês | Brazil | pt_BR |
Palavras-chave em inglês | DNA barcodes | pt_BR |
Palavras-chave em inglês | Phlebotominae | pt_BR |
Palavras-chave em inglês | phylogenetics | pt_BR |
Palavras-chave em inglês | sandfly species | pt_BR |
Palavras-chave em inglês | delimitation species | pt_BR |
Palavras-chave em inglês | complex taxonom | pt_BR |
Data de embargo | 2060-01-01 | |