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A DEEP INSIGHT INTO THE SIALOME OF RHODNIUS NEGLECTUS, A VECTOR OF CHAGAS DISEASE
Espectrometria de Massas
Dados de Sequência Molecular
Peptídeo Hidrolases
Proteínas e Peptídeos Salivares
Insetos Vetores
Rhodnius
Análise de Sequência de DNA
Autor
Santiago, Paula Beatriz
Assumpção, Teresa C. F.
Araújo, Carla Nunes de
Bastos, Izabela Marques Dourado
Neves, David
Silva, Ionizete Garcia da
Charneau, Sébastien
Queiroz, Rayner Myr L.
Raiol, Tainá
Oliveira, João Victor de Araújo
Sousa, Marcelo Valle de
Calvo, Eric
Ribeiro, José M. C.
Santana, Jaime M.
Assumpção, Teresa C. F.
Araújo, Carla Nunes de
Bastos, Izabela Marques Dourado
Neves, David
Silva, Ionizete Garcia da
Charneau, Sébastien
Queiroz, Rayner Myr L.
Raiol, Tainá
Oliveira, João Victor de Araújo
Sousa, Marcelo Valle de
Calvo, Eric
Ribeiro, José M. C.
Santana, Jaime M.
Afiliación
University of Brasília. Department of Cell Biology. Brasília, DF, Brazil.
University of Brasília. Department of Cell Biology. Brasília, DF, Brazil / National Institute of Allergy and Infectious Disease. Laboratory of Malaria and Vector Research. Vector Biology Section. Rockville, MA, United States of America.
University of Brasília. Department of Cell Biology. Brasília, DF, Brazil / University of Brasília. Ceilândia Faculty. Brasília, DF, Brazil.
University of Brasília. Department of Cell Biology. Brasília, DF, Brazil.
University of Brasília. Department of Cell Biology. Brasília, DF, Brazil.
University of Goiás. Department of Parasitology. Jataí, GO, Brazil.
University of Brasília. Department of Cell Biology. Brasília, DF, Brazil.
University of Brasília. Department of Cell Biology. Brasília, DF, Brazil.
University of Brasília. Department of Cell Biology. Brasília, DF, Brazil / Fundação Oswaldo Cruz. Fiocruz Amazônas - Instituto Leônidas e Maria Deane. Manaus, AM, Brasil.
University of Brasília. Department of Computer Science. Brasília, DF, Brazil.
University of Brasília. Department of Cell Biology. Brasília, DF, Brazil.
National Institute of Allergy and Infectious Disease. Laboratory of Malaria and Vector Research. Vector Biology Section. Rockville, MA, United States of America.
National Institute of Allergy and Infectious Disease. Laboratory of Malaria and Vector Research. Vector Biology Section. Rockville, MA, United States of America.
University of Brasília. Department of Cell Biology. Brasília, DF, Brazil.
University of Brasília. Department of Cell Biology. Brasília, DF, Brazil / National Institute of Allergy and Infectious Disease. Laboratory of Malaria and Vector Research. Vector Biology Section. Rockville, MA, United States of America.
University of Brasília. Department of Cell Biology. Brasília, DF, Brazil / University of Brasília. Ceilândia Faculty. Brasília, DF, Brazil.
University of Brasília. Department of Cell Biology. Brasília, DF, Brazil.
University of Brasília. Department of Cell Biology. Brasília, DF, Brazil.
University of Goiás. Department of Parasitology. Jataí, GO, Brazil.
University of Brasília. Department of Cell Biology. Brasília, DF, Brazil.
University of Brasília. Department of Cell Biology. Brasília, DF, Brazil.
University of Brasília. Department of Cell Biology. Brasília, DF, Brazil / Fundação Oswaldo Cruz. Fiocruz Amazônas - Instituto Leônidas e Maria Deane. Manaus, AM, Brasil.
University of Brasília. Department of Computer Science. Brasília, DF, Brazil.
University of Brasília. Department of Cell Biology. Brasília, DF, Brazil.
National Institute of Allergy and Infectious Disease. Laboratory of Malaria and Vector Research. Vector Biology Section. Rockville, MA, United States of America.
National Institute of Allergy and Infectious Disease. Laboratory of Malaria and Vector Research. Vector Biology Section. Rockville, MA, United States of America.
University of Brasília. Department of Cell Biology. Brasília, DF, Brazil.
Resumen en ingles
Background: Triatomines are hematophagous insects that act as vectors of Chagas disease. Rhodnius neglectus is one of these kissing bugs found, contributing to the transmission of this American trypanosomiasis. The saliva of hematophagous arthropods contains bioactive molecules responsible for counteracting host haemostatic, inflammatory, and immune responses. Methods/Principal Findings: Next generation sequencing and mass spectrometry-based protein identification were performed to investigate the content of triatomine R. neglectus saliva. We deposited 4,230 coding DNA sequences (CDS) in GenBank. A set of 636 CDS of proteins of putative secretory nature was extracted from the assembled reads, 73 of them confirmed by proteomic analysis. The sialome of R. neglectus was characterized and serine protease transcripts detected. The presence of ubiquitous protein families was revealed, including lipocalins, serine protease inhibitors, and antigen-5. Metalloproteases, disintegrins, and odorant binding protein families were less abundant. Conclusions/Significance: The data presented improve our understanding of hematophagous arthropod sialomes, and aid in understanding hematophagy and the complex interplay among vectors and their vertebrate hosts.
DeCS
Doença de ChagasEspectrometria de Massas
Dados de Sequência Molecular
Peptídeo Hidrolases
Proteínas e Peptídeos Salivares
Insetos Vetores
Rhodnius
Análise de Sequência de DNA
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