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Autor | Araújo, Taís Bacelar Sacramento de | |
Autor | Rocha, Leonardo de Oliveira Siquara da | |
Autor | Vidal, Manuela Torres Andion | |
Autor | Coelho, Paulo Lucas Cerqueira | |
Autor | Reis, Mitermayer Galvão dos | |
Autor | Souza, Bruno Solano de Freitas | |
Autor | Soares, Milena Botelho Pereira | |
Autor | Pereira, Thiago Almeida | |
Autor | Coletta, Ricardo Della | |
Autor | Bezerra, Daniel Pereira | |
Autor | Dias, Rosane Borges | |
Autor | Rocha, Clarissa Araújo Gurgel | |
Fecha de acceso | 2020-09-10T17:19:50Z | |
Fecha de disponibilización | 2020-09-10T17:19:50Z | |
Fecha de publicación | 2020 | |
Referencia | ARAÚJO, Taís Bacelar Sacramento de et al. GANT61 Reduces Hedgehog Molecule (GLI1) Expression and Promotes Apoptosis in Metastatic Oral Squamous Cell Carcinoma Cells. International Journal of Molecular Sciences, 2020. | pt_BR |
ISSN | 1661-6596 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/43301 | |
Promoción | Brazilian research funding agencies: Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq-309380/2015-4) and Fundação de Amparo à Pesquisa do Estado da Bahia (FAPESB-APP0006/2016). | pt_BR |
Idioma | eng | pt_BR |
Editor | MDPI | pt_BR |
Derechos de autor | open access | pt_BR |
Palabras clave en Portugués | Neoplasias orais | pt_BR |
Palabras clave en Portugués | Hedgehog | pt_BR |
Palabras clave en Portugués | Inibidores | pt_BR |
Palabras clave en Portugués | Câncer | pt_BR |
Título | GANT61 Reduces Hedgehog Molecule (GLI1) Expression and Promotes Apoptosis in Metastatic Oral Squamous Cell Carcinoma Cells | pt_BR |
Tipo del documento | Article | pt_BR |
Resumen en Inglés | Due to its importance in the pathogenesis of oral squamous cell carcinoma (OSCC), the Hedgehog (HH) pathway is considered a potential therapeutic target. We investigated the e ects of GANT61, a GLI inhibitor, on HH gene expression, as well as on metastatic OSCC cell proliferation and death. Following culture in DMEM medium, cytotoxicity of GANT61 against di erent tumor and non-tumor cell types was assessed by alamarBlue assays. Cytotoxicity analysis revealed that the metastatic HSC3 cell line was the most sensitive (IC50: 36 M) to the tested compound. The compound’s e ects on the expression of HH pathways components were analyzed by qPCR and Western blot; cell viability was analyzed by trypan blue assay and flow cytometry were used to investigate cell cycle phase, morphology, and death patterns in HSC3 cells. A significant reduction in mRNA levels of the GLI1 transcription factor was found after 12 h of treatment withGANT61. Protein expression levels of other HH pathway components (PTCH1, SHH, and Gli1) and HSC3 cell viability also decreased after 24 h of treatment. Cell cycle analysis and death pattern evaluations revealed significantly increased nuclear fragmentation in sub-G1 phase, as well as cell death due to apoptosis. In conclusion, the significantly reduced GLI1 gene expression seen in response to the GLI inhibitor indicates diminished downstream activation in HH pathway components. GANT61 significantly reduced cell viability in the metastatic cell line of OSCC and promoted a significant increase in nuclear fragmentation and cell death by apoptosis. | pt_BR |
Afiliación | Fundação Oswaldo Cruz. instituto Gonçalo Moniz. Salvador, BA, Brasil / Federal University of Bahia. Faculty of Dentistry. Department of Propedeutics. Salvador, BA, Brazil. | pt_BR |
Afiliación | Fundação Oswaldo Cruz. instituto Gonçalo Moniz. Salvador, BA, Brasil / Federal University of Bahia. Faculty of Dentistry. Department of Propedeutics. Salvador, BA, Brazil. | pt_BR |
Afiliación | Fundação Oswaldo Cruz. instituto Gonçalo Moniz. Salvador, BA, Brasil / Federal University of Bahia. Faculty of Dentistry. Department of Propedeutics. Salvador, BA, Brazil. | pt_BR |
Afiliación | Fundação Oswaldo Cruz. instituto Gonçalo Moniz. Salvador, BA, Brasil. | pt_BR |
Afiliación | Fundação Oswaldo Cruz. instituto Gonçalo Moniz. Salvador, BA, Brasil / Federal University of Bahia. School of Medicine. Department of Pathology. Salvador, BA, Brazil. | pt_BR |
Afiliación | Fundação Oswaldo Cruz. instituto Gonçalo Moniz. Salvador, BA, Brasil / Sao Rafael Hospital. D’Or Institute for Research and Education. Salvador, BA, Brazil. | pt_BR |
Afiliación | Fundação Oswaldo Cruz. instituto Gonçalo Moniz. Salvador, BA, Brasil. | pt_BR |
Afiliación | Stanford University School of Medicine. Institute for Stem Cell Biology and Regenerative Medicine. Stanford, CA, USA. | pt_BR |
Afiliación | University of Campinas. School of Dentistry. Department of Oral Diagnosis. Piracicaba, SP, Brazil. | pt_BR |
Afiliación | Fundação Oswaldo Cruz. instituto Gonçalo Moniz. Salvador, BA, Brasil. | pt_BR |
Afiliación | Fundação Oswaldo Cruz. instituto Gonçalo Moniz. Salvador, BA, Brasil / Federal University of Bahia. Faculty of Dentistry. Department of Propedeutics. Salvador, BA, Brazil. | pt_BR |
Afiliación | Fundação Oswaldo Cruz. instituto Gonçalo Moniz. Salvador, BA, Brasil / Federal University of Bahia. Faculty of Dentistry. Department of Propedeutics. Salvador, BA, Brazil / Federal University of Bahia. School of Medicine. Department of Pathology. Salvador, BA, Brazil. | pt_BR |
Palavras clave en Inglês | Oral neoplasms | pt_BR |
Palavras clave en Inglês | Hedgehog signaling pathway | pt_BR |
Palavras clave en Inglês | Hedgehog signaling inhibitors | pt_BR |
Palavras clave en Inglês | Targeted cancer therapy | pt_BR |