Author | Villarreal, Cristiane F. | |
Author | Santos, Dourivaldo S. | |
Author | Lauria, Pedro S. S. | |
Author | Gama, Kelly B. | |
Author | Espírito-Santo, Renan F. | |
Author | Juiz, Paulo J. L. | |
Author | Alves, Clayton Q. | |
Author | David, Jorge M. | |
Author | Soares, Milena Botelho Pereira | |
Access date | 2020-09-17T17:23:20Z | |
Available date | 2020-09-17T17:23:20Z | |
Document date | 2020 | |
Citation | VILLARREAL, Cristiane F. et al. Bergenin Reduces Experimental Painful Diabetic Neuropathy by Restoring Redox and Immune Homeostasis in the Nervous System. International Journal Molecular Science, 2020. | pt_BR |
ISSN | 1661-6596 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/43434 | |
Sponsorship | Conselho Nacional de Desenvolvimento Científico e Tecnológico,
grant number INCT/Rennofito 465536/2014-0. Funds to cover publication costs were provided by Fundação
Oswaldo Cruz. | pt_BR |
Language | eng | pt_BR |
Publisher | MDPI | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | Dor neuropática | pt_BR |
Subject in Portuguese | Neuropatia diabética | pt_BR |
Subject in Portuguese | Produto natural | pt_BR |
Subject in Portuguese | Analgésico | pt_BR |
Subject in Portuguese | Antioxidante | pt_BR |
Subject in Portuguese | Imunomodulação | pt_BR |
Subject in Portuguese | Citocina | pt_BR |
Subject in Portuguese | Medula espinhal | pt_BR |
Subject in Portuguese | Nervo ciático | pt_BR |
Title | Bergenin Reduces Experimental Painful Diabetic Neuropathy by Restoring Redox and Immune Homeostasis in the Nervous System | pt_BR |
Type | Article | pt_BR |
Abstract | Diabetic neuropathy is a frequent complication of diabetes. Symptoms include neuropathic
pain and sensory alterations—no e ective treatments are currently available. This work characterized
the therapeutic e ect of bergenin in a mouse (C57/BL6) model of streptozotocin-induced painful
diabetic neuropathy. Nociceptive thresholds were assessed by the von Frey test. Cytokines, antioxidant
genes, and oxidative stress markers were measured in nervous tissues by ELISA, RT-qPCR,
and biochemical analyses. Single (3.125–25 mg/kg) or multiple (25 mg/kg; twice a day for 14 days)
treatments with bergenin reduced the behavioral signs of diabetic neuropathy in mice. Bergenin
reduced both nitric oxide (NO) production in vitro and malondialdehyde (MDA)/nitrite amounts
in vivo. These antioxidant properties can be attributed to the modulation of gene expression by the
downregulation of inducible nitric oxide synthase (iNOS) and upregulation of glutathione peroxidase
and Nrf2 in the nervous system. Bergenin also modulated the pro- and anti-inflammatory cytokines
production in neuropathic mice. The long-lasting antinociceptive e ect induced by bergenin in
neuropathic mice, was associated with a shift of the cytokine balance toward anti-inflammatory
predominance and upregulation of antioxidant pathways, favoring the reestablishment of redox
and immune homeostasis in the nervous system. These results point to the therapeutic potential of
bergenin in the treatment of painful diabetic neuropathy. | pt_BR |
Affilliation | Universidade Federal da Bahia. Faculdade de Farmácia. Salvador, BA, Brasil / Fundação Oswaldo Cruz. instituto Gonçalo Moniz. Salvador, BA, Brasil. | pt_BR |
Affilliation | Universidade Federal da Bahia. Faculdade de Farmácia. Salvador, BA, Brasil. | pt_BR |
Affilliation | Universidade Federal da Bahia. Faculdade de Farmácia. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. instituto Gonçalo Moniz. Salvador, BA, Brasil. | pt_BR |
Affilliation | Universidade Federal da Bahia. Faculdade de Farmácia. Salvador, BA, Brasil / Fundação Oswaldo Cruz. instituto Gonçalo Moniz. Salvador, BA, Brasil. | pt_BR |
Affilliation | Universidade Federal do Recôncavo da Bahia. Feira de Santana, BA, Brazil. | pt_BR |
Affilliation | Universidade Estadual de Feira de Santana. Departamento de Ciências Exatas. Feira de Santana, BA, Brasil. | pt_BR |
Affilliation | , Universidade Federal da Bahia.mInstituto de Química. Salvador, BA, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. instituto Gonçalo Moniz. Salvador, BA, Brasil. | pt_BR |
Subject | Neuropathic pain | pt_BR |
Subject | Diabetic neuropathy | pt_BR |
Subject | Natural product | pt_BR |
Subject | Analgesic | pt_BR |
Subject | Antioxidant | pt_BR |
Subject | Immunomodulation | pt_BR |
Subject | Cytokine | pt_BR |
Subject | Spinal cord; | pt_BR |
Subject | Sciatic nerve | pt_BR |