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ArtigoDireito Autoral
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- IOC - Artigos de Periódicos [12502]
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KINETICS OF ANTIGEN SPECIFIC AND NON-SPECIFIC POLYCLONAL B-CELL RESPONSES DURING LETHAL PLASMODIUM YOELII MALARIA
Plasmodium yoelii
Antígenos
Ativação de células B policlonais
Células formadoras de placas
Plasmodium yoelii
Polyclonal B-cell activation
Plaque forming cells
Antigen
Afiliação
Groupe Hospitalier Pitié-Salpétriere. INSERM U 313. Paris, France.
Hôpital St-louis. Paris, France,
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Imunologia. Rio de Janeiro, RJ, Brasil.
Hôpital St-louis. Paris, France,
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Imunologia. Rio de Janeiro, RJ, Brasil.
Resumo em Inglês
In order to study the kinetics and composition of the polyclonal B-cell activation associated to malaria infection, antigen-specific and non-specific B-cell responses were evaluated in the spleens of mice infected with Plasmodium yoelii 17 XL or injected with lysed erythrocytes or plasma from P. yoelii infected mice or with P. falciparum culture supernatants. Spleen/body weigth ratio, numbers of nucleated spleen cells and Immunoglobulin-containing and Immunoglobulin-secreting cells increased progressively during the course of infection,in parallel to the parasitemia. A different pattern of kinetics was observed when anti-sheep red blood cell and anti-trinitrophenylated-sheep red blood cell plaque forming cells response were studied: maximum values were observed at early stages of infection, whereas the number of total Immunoglobulin-containing and Immunoglobulin-secreting cells were not yet altered. Conversely, at the end of infection, when these latter values reached their maximum, the anti-sheep red blood cell and anti-trinitrophenylated-sheep red blood cell specific responses were normal or even infranormal. In mice injected with Plasmodium-derived material, a higher increase in antigen-specific PFC was observed, as compared to the increase of Immunoglobulin-containing and Immunoglobulin-secreting cell numbers. This suggested a "preferential" (antigen-plus mitogen-induced) stimulation of antigen-specific cells rather than a generalized non-specific (mitogen-induced) triggering of B-lymphocytes. On the basis of these and previous results, it is suggested that polyclonal B-cell activation that takes place during the course of infection appears as a result of successive waves of antigen-specific B-cell activation.
Palavras-chave
MaláriaPlasmodium yoelii
Antígenos
Ativação de células B policlonais
Células formadoras de placas
Palavras-chave em inglês
MalariaPlasmodium yoelii
Polyclonal B-cell activation
Plaque forming cells
Antigen
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