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Autor | Rocha, Vinícius Pinto Costa | |
Autor | Dacher, Mariko | |
Autor | Young, Simon Alan | |
Autor | Kolokousi, Foteini | |
Autor | Efstathiou, Antonia | |
Autor | Späth, Gerald Frank | |
Autor | Soares, Milena Botelho Pereira | |
Autor | Smirlis, Despina | |
Fecha de acceso | 2020-09-22T16:46:25Z | |
Fecha de disponibilización | 2020-09-22T16:46:25Z | |
Fecha de publicación | 2020 | |
Referencia | ROCHA, Vinícius Pinto et al. CostaLeishmania dual-specificity tyrosine-regulated kinase 1 (DYRK1) is required for sustaining Leishmania stationary phase phenotype. Molecular Microbiology, p. 1-21, 2020. | pt_BR |
ISSN | 0950-382X | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/43539 | |
Promoción | International Division of the Institute Pasteur, Grant/Award Number: ACIP/ A13-2013; FAPESB/CAPES, Grant/Award Number: # PET0042/2013; Financiadora de Estudos e Projetos, Grant/Award Number: 02.13.0137.00; European Union and the National Ministry of Education and Religion Affairs under the Operational Strategic Reference Framework (NSRF 2007-2013) and the General Secretariat of Research and Technology (GSRT), Grant/Award Number: Action “KRIPIS I” (MIS 450598 | pt_BR |
Idioma | eng | pt_BR |
Editor | Wiley | pt_BR |
Derechos de autor | restricted access | pt_BR |
Palabras clave en Portugués | DYRK1 | pt_BR |
Palabras clave en Portugués | Crescimento | pt_BR |
Palabras clave en Portugués | Quinase | pt_BR |
Palabras clave en Portugués | Leishmania | pt_BR |
Palabras clave en Portugués | Estacionário | pt_BR |
Título | Leishmania dual-specificity tyrosine-regulated kinase 1 (DYRK1) is required for sustaining Leishmania stationary phase phenotype | pt_BR |
Tipo del documento | Article | pt_BR |
DOI | 10.1111/mmi.14464 | pt_BR |
Resumen en Inglés | Although the multiplicative and growth-arrested states play key roles in Leishmania development, the regulators of these transitions are largely unknown. In an attempt to gain a better understanding of these processes, we characterised one member of a family of protein kinases with dual specificity, LinDYRK1, which acts as a stasis regulator in other organisms. LinDYRK1 overexpressing parasites displayed a decrease in proliferation and in cell cycle re-entry of arrested cells. Parasites lacking LinDYRK1 displayed distinct fitness phenotypes in logarithmic and stationary growth phases. In logarithmic growth phase, LinDYRK1–/– parasites proliferated better than control lines, supporting a role of this kinase in stasis, while in stationary growth phase, LinDYRK1–/– p arasites h ad i mportant d efects a s t hey r ounded u p, a ccumulated vacuoles and lipid bodies and displayed subtle but consistent differences in lipid composition. Moreover, they expressed less metacyclic-enriched transcripts, displayed increased sensitivity to complement lysis and a significant reduction in survival within peritoneal macrophages. The distinct LinDYRK1–/– g rowth p hase p henotypes were mirrored by the distinct LinDYRK1 localisations in logarithmic (mainly in flagellar pocket area and endosomes) and late stationary phase (mitochondrion). Overall, this work provides first evidence for the role of a DYRK family member in sustaining promastigote stationary phase phenotype and infectivity. | pt_BR |
Afiliación | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Hospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, Brasil. | pt_BR |
Afiliación | Institut Pasteur and INSERM. Unité de Parasitologie Moléculaire et Signalisation. Department of Parasites and Insect Vectors. Paris, France. | pt_BR |
Afiliación | The University of St. Andrews. School of Biology. Biomedical Sciences Research Complex. St. Andrews, UK. | pt_BR |
Afiliación | Hellenic Pasteur Institute. Molecular Parasitology Laboratory, Microbiology Department. Athens, Greece. | pt_BR |
Afiliación | Hellenic Pasteur Institute. Molecular Parasitology Laboratory, Microbiology Department. Athens, Greece. | pt_BR |
Afiliación | Institut Pasteur and INSERM. Unité de Parasitologie Moléculaire et Signalisation. Department of Parasites and Insect Vectors. Paris, France. | pt_BR |
Afiliación | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Hospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, Brasil. | pt_BR |
Afiliación | Hellenic Pasteur Institute. Molecular Parasitology Laboratory, Microbiology Department. Athens, Greece. | pt_BR |
Palavras clave en Inglês | DYRK1 | pt_BR |
Palavras clave en Inglês | Growth | pt_BR |
Palavras clave en Inglês | Kinase | pt_BR |
Palavras clave en Inglês | Leishmania | pt_BR |
Palavras clave en Inglês | Stationary | pt_BR |