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ISOLATION OF A FRAGMENT HOMOLOGOUS TO THE RP49 CONSTITUTIVE GENE OF DROSOPHILA IN THE NEOTROPICAL MALARIA VECTOR ANOPHELES AQUASALIS (DIPTERA: CULICIDAE)
Aedes aegypti
Culex quinquefasciatus
Drosophila constitutive gene
rp49
rpL32
Mosquitos
Aedes aegypti
Culex quinquefasciatus
Drosophila constitutive gene
rp49
rpL32
Mosquitoes
Afiliación
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Entomologia. Rio de Janeiro, RJ, Brasil / Instituto de Biologia do Exército. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Entomologia. Rio de Janeiro, RJ, Brasil / Instituto de Biologia do Exército. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil.
Resumen en ingles
The constitutive ribosomal gene rp49 is frequently used as an endogenous control in Drosophila gene expression
experiments. Using the degenerate primer PCR technique we have cloned a fragment homologous to this gene in
Anopheles aquasalis Curry, a Neotropical vector of malaria. In addition, based on this first sequence, a new primer
was designed, which allowed the isolation of fragments of rp49 in two other species, Aedes aegypti (Linnaeus) and
Culex quinquefasciatus Say, suggesting that it could be used to clone fragments of this gene in a number of other
mosquito species. Primers were also designed to specifically amplify rp49 cDNA fragments in An. aquasalis and Ae.
aegypti, showing that rp49 could be used as a good constitutive control in gene expression studies of these and other
vectorially important mosquito species.
Palabras clave en portugues
Anopheles aquasalisAedes aegypti
Culex quinquefasciatus
Drosophila constitutive gene
rp49
rpL32
Mosquitos
Palabras clave en ingles
Anopheles aquasalisAedes aegypti
Culex quinquefasciatus
Drosophila constitutive gene
rp49
rpL32
Mosquitoes
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