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https://www.arca.fiocruz.br/handle/icict/45557
IN VITRO AND IN VIVO IMMUNOMODULATORY ACTIVITY OF PHYSALIS ANGULATA CONCENTRATED ETHANOLIC EXTRACT
Author
Affilliation
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.
Fundação Oswaldo Cruz. Farmanguinhos. Laboratório de Química de Produtos Naturais-PN2- Extração, Isolamento e Purificação. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Farmanguinhos. Laboratório de Química de Produtos Naturais-PN2- Extração, Isolamento e Purificação. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.
Fundação Oswaldo Cruz. Farmanguinhos. Laboratório de Química de Produtos Naturais-PN2- Extração, Isolamento e Purificação. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Farmanguinhos. Laboratório de Química de Produtos Naturais-PN2- Extração, Isolamento e Purificação. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.
Abstract
The need for new immunomodulatory drugs is due to the side
effects associated with the prolonged use of the currently
used immunomodulatory drugs. In this context, the present
work aimed to investigate the immunomodulatory effect of
an ethanolic concentrated extract from Physalis angulata. The
cytotoxicity of samples was determined using peritoneal macrophages
though the Alamar Blue assay. The immunomodulatory
activity of the ethanolic extract from P. angulata on activated
macrophages was determined by measurement of
nitrite and cytokine production. The immunosuppressive effects
of the ethanolic extract from P. angulata was evaluated
on lymphocyte proliferation and cytokine production. The effects
of the extract on cell cycle progression and cell death on
lymphocytes were evaluated by flow cytometry. Lastly, the
ethanolic extract from P. angulata was tested in vivo in toxicological
tests and in models of peritonitis and delayed-type hypersensitivity
response. The ethanolic extract from P. angulata
decreased nitrite, interleukin-6, interleukin-12, and TNF-α
production by activated macrophages without affecting the
cell viability. In addition, the ethanolic extract from P. angulata
inhibited lymphoproliferation and the secretion of interleukin-
2, interleukin-6, and IFN-γ, and increased interleukin-4 secretion
by activated splenocytes. Flow cytometry analysis in lymphocyte
cultures showed that treatment with the ethanolic
extract from P. angulata induces cell cycle arrest in the G1
phase followed by cell death by apoptosis. Moreover, mice
treated with the extract from P. angulata at 100 or 200mg/kg
did not show signs of toxicity or alterations in serum components.
Finally, the ethanolic extract from P. angulata significantly
reduced neutrophil migration and reduced paw edema
in bovine serum albumin-induced the delayed-type hypersensitivity
responsemodel. Our results demonstrate the potential
of the ethanolic extract of P. angulata as an alternative for the
treatment of immune-inflammatory diseases.
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