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Autor | Daltro, Sérgio Ricardo Teixeira | |
Autor | Santos, Ivanilson Pimenta | |
Autor | Barros, Paula Ladeia | |
Autor | Moreira, Diogo Rodrigo Magalhães | |
Autor | Tomassini, Therezinha Coelho Barbosa | |
Autor | Ribeiro, Ivone Maria | |
Autor | Santos, Ricardo Ribeiro dos | |
Autor | Meira, Cássio Santana | |
Autor | Soares, Milena Botelho Pereira | |
Fecha de acceso | 2021-01-11T12:57:24Z | |
Fecha de disponibilización | 2021-01-11T12:57:24Z | |
Fecha de publicación | 2020 | |
Referencia | DALTRO, Sérgio Ricardo Teixeira et al. In vitro and In Vivo Immunomodulatory Activity of Physalis angulata Concentrated Ethanolic Extract. Planta Médica, 2020. | pt_BR |
ISSN | 0032-0943 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/45557 | |
Promoción | CNPq (grant number 562655/ 2010-7), PRONEX (grant number 0002/2014) and FAPESB (grant number 0042/2013) | pt_BR |
Idioma | eng | pt_BR |
Editor | Thieme Gruppe | pt_BR |
Derechos de autor | open access | pt_BR |
Palabras clave en Portugués | Função linfocitária | pt_BR |
Palabras clave en Portugués | Physalis angulata | pt_BR |
Palabras clave en Portugués | Solanaceae | pt_BR |
Palabras clave en Portugués | Ativação de macrófagos | pt_BR |
Título | In vitro and In Vivo Immunomodulatory Activity of Physalis angulata Concentrated Ethanolic Extract | pt_BR |
Tipo del documento | Article | pt_BR |
DOI | 10.1055/a-1237-4268 | |
Resumen en Inglés | The need for new immunomodulatory drugs is due to the side effects associated with the prolonged use of the currently used immunomodulatory drugs. In this context, the present work aimed to investigate the immunomodulatory effect of an ethanolic concentrated extract from Physalis angulata. The cytotoxicity of samples was determined using peritoneal macrophages though the Alamar Blue assay. The immunomodulatory activity of the ethanolic extract from P. angulata on activated macrophages was determined by measurement of nitrite and cytokine production. The immunosuppressive effects of the ethanolic extract from P. angulata was evaluated on lymphocyte proliferation and cytokine production. The effects of the extract on cell cycle progression and cell death on lymphocytes were evaluated by flow cytometry. Lastly, the ethanolic extract from P. angulata was tested in vivo in toxicological tests and in models of peritonitis and delayed-type hypersensitivity response. The ethanolic extract from P. angulata decreased nitrite, interleukin-6, interleukin-12, and TNF-α production by activated macrophages without affecting the cell viability. In addition, the ethanolic extract from P. angulata inhibited lymphoproliferation and the secretion of interleukin- 2, interleukin-6, and IFN-γ, and increased interleukin-4 secretion by activated splenocytes. Flow cytometry analysis in lymphocyte cultures showed that treatment with the ethanolic extract from P. angulata induces cell cycle arrest in the G1 phase followed by cell death by apoptosis. Moreover, mice treated with the extract from P. angulata at 100 or 200mg/kg did not show signs of toxicity or alterations in serum components. Finally, the ethanolic extract from P. angulata significantly reduced neutrophil migration and reduced paw edema in bovine serum albumin-induced the delayed-type hypersensitivity responsemodel. Our results demonstrate the potential of the ethanolic extract of P. angulata as an alternative for the treatment of immune-inflammatory diseases. | pt_BR |
Afiliación | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. | pt_BR |
Afiliación | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. | pt_BR |
Afiliación | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. | pt_BR |
Afiliación | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. | pt_BR |
Afiliación | Fundação Oswaldo Cruz. Farmanguinhos. Laboratório de Química de Produtos Naturais-PN2- Extração, Isolamento e Purificação. Rio de Janeiro, RJ, Brasil. | pt_BR |
Afiliación | Fundação Oswaldo Cruz. Farmanguinhos. Laboratório de Química de Produtos Naturais-PN2- Extração, Isolamento e Purificação. Rio de Janeiro, RJ, Brasil. | pt_BR |
Afiliación | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. | pt_BR |
Afiliación | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. | pt_BR |
Afiliación | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. | pt_BR |
Palavras clave en Inglês | lymphocyte function, | pt_BR |
Palavras clave en Inglês | Physalis angulata | pt_BR |
Palavras clave en Inglês | Solanaceae | pt_BR |
Palavras clave en Inglês | Macrophage activation | pt_BR |