Author | Bitu, Vanessa de Carvalho Nilo | |
Author | Pereira, José Henrique Alves | |
Author | Ferreira, Vinícius de Carvalho Nilo Bitu | |
Author | Ribeiro Filho, Jaime | |
Author | Vega, Maria Celeste | |
Author | Boligon, Aline Augusti | |
Author | Coutinho, Henrique Douglas Melo | |
Author | Menezes, Irwin Rose Alencar de | |
Access date | 2021-02-02T12:26:24Z | |
Available date | 2021-02-02T12:26:24Z | |
Document date | 2020 | |
Citation | BITU, Vanessa de Carvalho Nilo et al. Chemical Composition and In Vitro Antiprotozoal Properties of Cephaelis ipepacuanha. Brazilian Journal of health Review, Curitiba, v. 3, n. 6, p. 16525-16536, nov./dez.2020. | pt_BR |
ISSN | 2595-6825 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/45918 | |
Language | eng | pt_BR |
Publisher | Desconhecido | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | Cephaelis ipecacuanha | pt_BR |
Subject in Portuguese | Leishmania braziliensis | pt_BR |
Subject in Portuguese | Leishmania Infantum | pt_BR |
Subject in Portuguese | Trypanosoma Cruzi | pt_BR |
Subject in Portuguese | Biomarcadores Tumorais | pt_BR |
Subject in Portuguese | Antiprotozoária | pt_BR |
Title | Chemical Composition and In Vitro Antiprotozoal Properties of Cephaelis ipepacuanha | pt_BR |
Type | Article | pt_BR |
DOI | 10.34119/bjhrv3n6-074 | |
Abstract | Cephaelis ipecacuanha is a medicinal plant used in folk medicine for the treatment of amebiasis. However, the pharmacological properties of this species remain poorly understood.
Objective: Characterize chemical composition and in vitro antiprotozoal activity of Cephaelis ipecacuanha.
Method: The aqueous extract was lyophilized using the Chisrt Alpha apparatus and transferred to 96-well plates at concentrations ranging from 62.5 to 1000 μg / mL. The antiprotozoal activities against Trypanosoma cruzi, Leishmania brasiliensis, and L. infantum were evaluated after 72h incubation. Fibroblasts incubated at the same conditions for 24h were used as a cytotoxicity control. The readings were performed by spectrophotometry after staining with resazurin. The chemical composition of the extract was analyzed by High-Performance Liquid Chromatography (HPLC-DAD).
Results: The results demonstrated that C. ipecacuanha had moderate antiprotozoal activity against T. cruzi. However, its cytotoxicity against the fibroblasts was significantly higher.
Conclusion: This finding suggests that the use of this plant by the population, besides not having significant benefits, can cause associated health risks. Conclusão: Esse achado sugere que o uso dessa planta pela população, além de não apresentar benefícios significativos, pode acarretar riscos à saúde associados. | pt_BR |
Affilliation | Centro Universitário Dr. Leão Sampaio. Programa do Mestrado de Ensino em Saúde. Juazeiro do Norte, CE, Brasil. | pt_BR |
Affilliation | Centro Universitário Dr. Leão Sampaio. Programa do Mestrado de Ensino em Saúde. Juazeiro do Norte, CE, Brasil. | pt_BR |
Affilliation | Universidade do Estado do Mato Grosso. Cáceres, MG, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Bahia, Brasil. | pt_BR |
Affilliation | Fundación Moisés Bertoni. Centro para el Desarrollo de la Investigación Científica. Laboratorios Diaz Gill. Asuncion, Paraguay. | pt_BR |
Affilliation | Universidade Federal de Santa Maria. Santa Maria, RS, Brasil. | pt_BR |
Affilliation | Universidade Regional do Cariri. Crato, CE, Brasil. | pt_BR |
Affilliation | Universidade Regional do Cariri. Crato, CE, Brasil. | pt_BR |
Subject | Cephaelis ipecacuanha | pt_BR |
Subject | Leishmania braziliensis | pt_BR |
Subject | Leishmania Infantum | pt_BR |
Subject | Trypanosoma Cruzi | pt_BR |
Subject | Secondary metabolites. | pt_BR |
Subject | Antiprotozoal activity. | pt_BR |