Author | Jackson, Sandra | |
Author | Peret, Teresa C. T. | |
Author | Ziegler, Thedi T. | |
Author | Thornburg, Natalie J. | |
Author | Besselaar, Terry | |
Author | Broor, Shobha | |
Author | Barr, Ian | |
Author | Baumeister, Elsa | |
Author | Chadha, Mandeep | |
Author | Chittaganpitch, Malinee | |
Author | Darmaa, Badarch | |
Author | Ellis, Joanna | |
Author | Fasce, Rodrigo | |
Author | Hering, Belinda | |
Author | Herve, Kadjo | |
Author | Siddhivinayak, Hirve | |
Author | Li, Yan | |
Author | Pisareva, Maria | |
Author | Moen, Ana | |
Author | Naguib, Amel | |
Author | Palekar, Rakee | |
Author | Potdar, Vasha | |
Author | Siqueira, Marilda Agudo Mendonça Teixeira de | |
Author | Treurnicht, Florette | |
Author | Tivane, Almiro | |
Author | Venter, Marietjie | |
Author | Wairagkar, Niteen | |
Author | Zambom, Maria | |
Author | Zhang, Wenqing | |
Access date | 2021-02-21T14:03:19Z | |
Available date | 2021-02-21T14:03:19Z | |
Document date | 2020 | |
Citation | JACKSON, Sandra et al. Results from the WHO external quality assessment for the respiratory syncytial virus pilot, 2016-17. Influenza Other Respiratory Viruses, v. 14, p. 671-677, 2020. | pt_BR |
ISSN | 1750-2659 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/46122 | |
Language | eng | pt_BR |
Publisher | Wiley | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | Avaliação de qualidade externa RSV | pt_BR |
Subject in Portuguese | Reação em cadeia da polimerase | pt_BR |
Subject in Portuguese | Vírus sinciciais respiratórios | pt_BR |
Title | Results from the WHO external quality assessment for the respiratory syncytial virus pilot, 2016-17 | pt_BR |
Type | Article | pt_BR |
DOI | 10.1111/irv.12771 | |
Abstract | Background: External quality assessments (EQAs) for the molecular detection of respiratory
syncytial virus (RSV) are necessary to ensure the provision of reliable and accurate results. One of the objectives of the pilot of the World Health Organization
(WHO) Global RSV Surveillance, 2016-2017, was to evaluate and standardize RSV
molecular tests used by participating countries. This paper describes the first WHO
RSV EQA for the molecular detection of RSV.
Methods: The WHO implemented the pilot of Global RSV Surveillance based on the
WHO Global Influenza Surveillance and Response System (GISRS) from 2016 to 2018
in 14 countries. To ensure standardization of tests, 13 participating laboratories were
required to complete a 12 panel RSV EQA prepared and distributed by the Centers for
Disease Control and Prevention (CDC), USA. The 14th laboratory joined the pilot late
and participated in a separate EQA. Laboratories evaluated a RSV rRT-PCR assay developed
by CDC and compared where applicable, other Laboratory Developed Tests
(LDTs) or commercial assays already in use at their laboratories.
Results: Laboratories performed well using the CDC RSV rRT-PCR in comparison with
LDTs and commercial assays. Using the CDC assay, 11 of 13 laboratories reported correct
results. Two laboratories each reported one false-positive finding. Of the laboratories
using LDTs or commercial assays, results as assessed by Ct values were 100% correct
for 1/5 (20%). With corrective actions, all laboratories achieved satisfactory outputs.
Conclusions: These findings indicate that reliable results can be expected from
this pilot. Continued participation in EQAs for the molecular detection of RSV is
recommended. | pt_BR |
Affilliation | Global Influenza Programme, World Health Organization. Geneva, Switzerland. | pt_BR |
Affilliation | Division of Viral Diseases, Respiratory Viruses Branch, Centers for Disease Control and Prevention (CDC). Atlanta, GA, USA. | pt_BR |
Affilliation | Research Center for Child Psychiatry, University of Turku. Turku, Finland. | pt_BR |
Affilliation | Division of Viral Diseases, Respiratory Viruses Branch, Centers for Disease Control and Prevention (CDC). Atlanta, GA, USA. | pt_BR |
Affilliation | Global Influenza Programme, World Health Organization. Geneva, Switzerland. | pt_BR |
Affilliation | Medicine and Health Sciences, Shree Guru Gobind Singh Tricentenaryl University. Gurugram, India. | pt_BR |
Affilliation | Victorian Infectious Diseases Reference Laboratory, Peter Doherty Institute for Infection and Immunity. Melbourne, VIC, Australia. | pt_BR |
Affilliation | Departamento Virologia, INEI-ANLIS Carlos G Malbrán, Buenos Aires, Argentina, | pt_BR |
Affilliation | National Institute of Virology, Indian Council of Medical Research. Pune, India. | pt_BR |
Affilliation | Department of Medical Sciences, Ministry of Public Health. Nonthaburi, Thailand. | pt_BR |
Affilliation | Virology Laboratory, National Center for Communicable Diseases. Ulanbaatar, Mongolia. | pt_BR |
Affilliation | Virus Reference Department, Public Health England. London, UK. | pt_BR |
Affilliation | Sub-department of Viral Diseases, Instituto de Salud Pública de Chile. Santiago, Chile. | pt_BR |
Affilliation | African Region Office, World Health Organization., Brazzaville, Republic of Congo. | pt_BR |
Affilliation | Department of Epidemic Viruses, Institut Pasteur de Côte d’Ivoire. Abidjan, Côte d’Ivoire. | pt_BR |
Affilliation | Global Influenza Programme, World Health Organization. Geneva, Switzerland. | pt_BR |
Affilliation | Influenza and Respiratory Viruses Section, National Microbiology Laboratory, Public Health Agency of Canada. Winnipeg, MB, Canada. | pt_BR |
Affilliation | Laboratory of Molecular Virology, Smorodintsev Research Institute of Influenza. St. Petersburg, Russian Federation. | pt_BR |
Affilliation | Global Influenza Programme, World Health Organization. Geneva, Switzerland. | pt_BR |
Affilliation | Central Public Health Laboratory, Ministry of Health. Cairo, Egypt. | pt_BR |
Affilliation | Pan American Health Organization. Washington, DC, USA. | pt_BR |
Affilliation | National Institute of Virology, Indian Council of Medical Research. Pune, India. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Centre for Respiratory Diseases and Meningitis, National Institute for Communicable Diseases. Johannesburg, South Africa | pt_BR |
Affilliation | Laboratório de Isolamento Viral, Instituto Nacional de Saúde.. Maputo, Mozambique | pt_BR |
Affilliation | Center for Viral Zoonosis, Department of Medical Virology.University of Pretoria, Pretoria, South Africa. | pt_BR |
Affilliation | Bill and Melinda Gates Foundation. Seattle, WA, USA. | pt_BR |
Affilliation | Virus Reference Department, Public Health England. London, UK. | pt_BR |
Affilliation | Global Influenza Programme, World Health Organization. Geneva, Switzerland. | pt_BR |
Subject | RSV external quality assessment | pt_BR |
Subject | Polymerase chain reaction | pt_BR |
Subject | Respiratory syncytial viruses | pt_BR |