Author | Santos, Catiule de Oliveira | |
Author | Costa, Sidnei Ferro | |
Author | Souza, Fabiana Santana | |
Author | Mendes, Jessica Mariane Ferreira | |
Author | Pinheiro, Cristiane Garboggini Melo de | |
Author | Moreira, Diogo Rodrigo de Magalhães | |
Author | Silva, Luciano Kalabric | |
Author | Lima, Valeria Marçal Felix de | |
Author | Oliveira, Geraldo Gileno de Sá | |
Access date | 2021-03-08T12:26:01Z | |
Available date | 2021-03-08T12:26:01Z | |
Document date | 2021 | |
Citation | SANTOS, Catiule de Oliveira et al. Blocking IL-10 signaling with soluble IL-10 receptor restores in vitro specific lymphoproliferative response in dogs with leishmaniasis caused by Leishmania infantum. Plos One, 2021. | pt_BR |
ISSN | 1932-6203 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/46290 | |
Sponsorship | Program for Technological Development of Tools for Health
(PDTIS-FIOCRUZ) for the use of its facilities and to the Postgraduate Course of Biotechnology
in Health and Investigative Medicine. | pt_BR |
Language | eng | pt_BR |
Publisher | Public Library of Science | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | Leishmaniose | pt_BR |
Subject in Portuguese | Cães | pt_BR |
Subject in Portuguese | Interleucina-10 | pt_BR |
Subject in Portuguese | Leishmania infantum | pt_BR |
Subject in Portuguese | Técnicas In Vitro | pt_BR |
Title | Blocking IL-10 signaling with soluble IL-10 receptor restores in vitro specific lymphoproliferative response in dogs with leishmaniasis caused by Leishmania infantum | pt_BR |
Type | Article | pt_BR |
DOI | 10.1371/journal.pone.0239171 | |
Abstract | rIL-10 plays a major role in restricting exaggerated inflammatory and immune responses,
thus preventing tissue damage. However, the restriction of inflammatory and immune
responses by IL-10 can also favor the development and/or persistence of chronic infections
or neoplasms. Dogs that succumb to canine leishmaniasis (CanL) caused by L. infantum
develop exhaustion of T lymphocytes and are unable to mount appropriate cellular immune
responses to control the infection. These animals fail to mount specific lymphoproliferative
responses and produce interferon gamma and TNF-alpha that would activate macrophages
and promote destruction of intracellular parasites. Blocking IL-10 signaling may contribute
to the treatment of CanL. In order to obtain a tool for this blockage, the present work endeavored
to identify the canine casIL-10R1 amino acid sequence, generate a recombinant baculovirus
chromosome encoding this molecule, which was expressed in insect cells and
subsequently purified to obtain rcasIL-10R1. In addition, rcasIL-10R1 was able to bind to
homologous IL-10 and block IL-10 signaling pathway, as well as to promote lymphoproliferation
in dogs with leishmaniasis caused by L. infantum. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Patologia Estrutural e Molecular. Laboratório de Engenharia Tecidual e Imunofarmacologia. Laboratório de Patologia e Biologia Molecular. Salvador, BA, Bahia, Brasil. | pt_BR |
Affilliation | São Paulo State University. School of Veterinary Medicine. Department of Clinical Medicine, Surgery and Animal Reproduction. Araçatuba, SP, Brazil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Patologia Estrutural e Molecular. Laboratório de Engenharia Tecidual e Imunofarmacologia. Laboratório de Patologia e Biologia Molecular. Salvador, BA, Bahia, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Patologia Estrutural e Molecular. Laboratório de Engenharia Tecidual e Imunofarmacologia. Laboratório de Patologia e Biologia Molecular. . Salvador, BA, Bahia, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Patologia Estrutural e Molecular. Laboratório de Engenharia Tecidual e Imunofarmacologia. Laboratório de Patologia e Biologia Molecular. . Salvador, BA, Bahia, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Patologia Estrutural e Molecular. Laboratório de Engenharia Tecidual e Imunofarmacologia. Laboratório de Patologia e Biologia Molecular. . Salvador, BA, Bahia, Brasil. | pt_BR |
Affilliation | São Paulo State University. School of Veterinary Medicine. Department of Clinical Medicine, Surgery and Animal Reproduction. Araçatuba, SP, Brazil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Patologia Estrutural e Molecular. Laboratório de Engenharia Tecidual e Imunofarmacologia. Laboratório de Patologia e Biologia Molecular. . Salvador, BA, Bahia, Brasil. | pt_BR |
Subject | Leishmaniasis | pt_BR |
Subject | Dogs | pt_BR |
Subject | Interleukin-10 | pt_BR |
Subject | Leishmania infantum | pt_BR |
Subject | In Vitro Techniques | pt_BR |