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FIELD APPLICATION OF NIR SPECTROSCOPY FOR THE DISCRIMINATION OF THE BIOMPHALARIA SPECIES THAT ARE INTERMEDIATE HOSTS OF SCHISTOSOMA MANSONI IN BRAZIL
Técnica inovadora
Molusco
Caracóis de água doce
Fenótipo químico
Esquistossomose
Innovative technique
Mollusk
Freshwater snails
Chemical phenotype
Schistosomiasis
Autor
Afiliación
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Monitoramento e Prevenção da Saúde Ambiental. Rio de Janeiro, RJ, Brasil.
Universidade Estadual de Campinas. Instituto de Química. Campinas, SP, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Malacologia. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Malacologia. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Monitoramento e Prevenção da Saúde Ambiental. Rio de Janeiro, RJ, Brasil.
Universidade Estadual de Campinas. Instituto de Química. Campinas, SP, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Malacologia. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Malacologia. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Monitoramento e Prevenção da Saúde Ambiental. Rio de Janeiro, RJ, Brasil.
Resumen en ingles
Near Infrared Spectroscopy (NIRS) is a spectroscopic technique that evaluates the
vibrational energy levels of the chemical bonds of molecules within a wavelength range
of 750–2,500 nm. This simple method acquires spectra that provide qualitative and
quantitative data on the chemical components of the biomass of living organisms
through the interaction between the electromagnetic waves and the sample. NIRS is an
innovative, rapid, and non-destructive technique that can contribute to the differentiation
of species based on their chemical phenotypes. Chemical profiles were obtained by
NIRS from three snail species (Biomphalaria glabrata, Biomphalaria straminea, and
Biomphalaria tenagophila) that are intermediate hosts of Schistosoma mansoni in Brazil.
The correct identification of these species is important from an epidemiological viewpoint,
given that each species has distinct biological and physiological characteristics. The
present study aimed to develop a chemometric model for the interspecific and intra specific classification of the three species, focusing on laboratory and field populations.
The data were obtained from 271 live animals, including 150 snails recently collected from
the field, with the remainder being raised in the laboratory. Populations were sampled at
three localities in the Brazilian state of Rio de Janeiro, in the municipalities of Sumidouro
(B. glabrata) and Paracambi (B. straminea), and the borough of Jacarepaguá in the Rio
de Janeiro city (B. tenagophila). The chemometric analysis was run in the Unscrambler®
software. The intra-specific classification of the field and laboratory populations obtained
accuracy rates of 72.5% (B. tenagophila), 77.5% (B. straminea), and 85.0% (B. glabrata).
The interspecific differentiation had a hit rate of 75% for the field populations and 80%
for the laboratory populations. The results indicate chemical and metabolic differences
between populations of the same species from the field and the laboratory. The chemical
phenotype, which is closely related to the metabolic profile of the snails, varied between environments. Overall, the NIRS technique proved to be a potentially valuable tool for
medical malacology, enabling the systematic discrimination of the Biomphalaria snails
that are the intermediate hosts of S. mansoni in Brazil.
Palabras clave en portugues
Espectroscopia infravermelha próximaTécnica inovadora
Molusco
Caracóis de água doce
Fenótipo químico
Esquistossomose
Palabras clave en ingles
Near infrared spectroscopyInnovative technique
Mollusk
Freshwater snails
Chemical phenotype
Schistosomiasis
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