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Autor | Roberto, Ana Emília M. | |
Autor | Xavier, Danilo E. | |
Autor | Vidal, Esteban E. | |
Autor | Vidal, Cláudia Fernanda de L. | |
Autor | Neves, Rejane P. | |
Autor | Lima-Neto, Reginaldo G. de | |
Fecha de acceso | 2021-05-13T16:03:57Z | |
Fecha de disponibilización | 2021-05-13T16:03:57Z | |
Fecha de publicación | 2020 | |
Referencia | ROBERTO, A. E. M. et al. Rapid Detection of Echinocandins Resistance by MALDI-TOF MS in Candida parapsilosis Complex. Microorganisms, v. 8, n. 1, 13 jan. 2020. | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/47156 | |
Descripción | The authors also would like to thank the Center for Strategic Technologies Northeastern (CETENE, MCTI, Brazil) for MALDI-TOF MS analyses and Hospital das Clínicas de Pernambuco (UFPE, Brazil) for providing the biological samples from the patients. | pt_BR |
Promoción | FACEPE | pt_BR |
Idioma | eng | pt_BR |
Derechos de autor | open access | pt_BR |
Título | Rapid Detection of Echinocandins Resistance by MALDI-TOF MS in Candida parapsilosis Complex | pt_BR |
Tipo del documento | Article | pt_BR |
DOI | 10.3390/microorganisms8010109 | |
Resumen en Inglés | Mass spectrometry by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) was used to identify and differentiate the pattern of susceptibility of clinical isolates of Candida parapsilosis complex. 17 C. parapsilosis sensu stricto, 2 C. orthopsilosis, and 1 C. metapsilosis strains were obtained from blood cultures, and three different inocula (103, 105, and 107 CFU/mL) were evaluated against three echinocandins at concentrations ranging from 0.03 to 16 µg/mL after incubation of 1 h, 2 h, and 3 h. Drug-free control was used. The spectra obtained at these concentrations were applied to generate composite correlation index (CCI) matrices for each yeast individually. After cross correlations and autocorrelations of each spectra with null (zero) and maximal (16) concentrations, the CCI was used as separation parameter among spectra. Incubation time and inoculum were critical factors to reach higher precision and reliability of this trial. With an incubation time of 3 h and inoculum of 107 CFU/mL, it was possible to determine the breakpoint of the clinical yeasts by MALDI-TOF that presented high agreement with the clinical laboratory standard institute (CLSI) reference method. Herein, we show that mass spectrometry using the MALDI-TOF technique is powerful when it exploits antifungal susceptibility testing assays. | pt_BR |
Afiliación | Universidade Federal de Pernambuco. Programa de Pós-Graduação em Biologia de Fungos. Recife, PE, Brasil. | pt_BR |
Afiliación | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil. | pt_BR |
Afiliación | Centro de Tecnologias Estratégicas do Nordeste. Recife, PE, Brasil. | pt_BR |
Afiliación | Hospital das Clínicas. Recife, PE, Brasil. | pt_BR |
Afiliación | Universidade Federal de Pernambuco. Programa de Pós-Graduação em Biologia de Fungos. Recife, PE, Brasil. | pt_BR |
Afiliación | Universidade Federal de Pernambuco. Programa de Pós-Graduação em Biologia de Fungos. Recife, PE, Brasil. | pt_BR |
Palavras clave en Inglês | mass spectrometry | pt_BR |
Palavras clave en Inglês | susceptibility testing | pt_BR |
Palavras clave en Inglês | candidemia | pt_BR |
Palavras clave en Inglês | resistance | pt_BR |
DeCS | Espectrometria de Massas | pt_BR |
DeCS | Candidemia | pt_BR |
DeCS | Candida parapsilosis | pt_BR |
DeCS | Equinocandinas | pt_BR |