Author | Carramaschi, Isabel N. | |
Author | Ferreira, Vítor dos S. B. | |
Author | Chagas, Thiago P.G. | |
Author | Corrêa, Lais L. | |
Author | Picão, Renata C, | |
Author | Queiroz, Margareth M. de C. | |
Author | Rangel, Karyne | |
Author | Jardim, Rodrigo | |
Author | Mota, Fabio F. da | |
Author | Zahner, Viviane | |
Access date | 2021-06-24T18:33:20Z | |
Available date | 2021-06-24T18:33:20Z | |
Document date | 2021 | |
Citation | CARRAMASCHI, Isabel N. et al. Multidrug-resistant Klebsiella quasipneumoniae subsp. similipneumoniae carrying blaNDM-blaCTX-M15 isolated from flies in Rio de Janeiro, Brazil. Journal of Global Antimicrobial Resistance, v. 24, p. 1-5, 2021. | pt_BR |
ISSN | 2213-7165 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/47859 | |
Language | eng | pt_BR |
Publisher | Science Direct | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | Klebsiella quasipneumoniae | pt_BR |
Subject in Portuguese | Moscas | pt_BR |
Subject in Portuguese | Saúde Pública | pt_BR |
Subject in Portuguese | Resistência a antibióticos | pt_BR |
Title | Multidrug-resistant Klebsiella quasipneumoniae subsp. similipneumoniae carrying blaNDM-blaCTX-M15 isolated from flies in Rio de Janeiro, Brazil | pt_BR |
Type | Article | pt_BR |
DOI | 10.1016/j.jgar.2020.11.021 | |
Abstract | Objectives: Flies have been implicated in the dispersal of medically important bacteria including
members of the genus Klebsiella between different environmental compartments. The aim of this study
was to retrieve and characterize antibiotic-resistant bacteria from flies collected near to hospitals.
Methods: Flies were collected in the vicinity of medical facilities and examined for bacteria demonstrating
phenotypic resistance to ceftriaxone, followed by determination of phenotypic and genotypic resistance
profiles. In addition, whole genome sequencing followed by phylogenetic analysis and resistance
genotyping were performed with the multidrug-resistant (MDR) strain Lemef23, identified as Klebsiella
quasipneumoniae subsp. similipneumoniae.
Results: The strain Lemef23, classified by multiple locus sequence typing as novel ST 3397, harboured
numerous resistance genes. The blaNDM was located on a Tn3000 element, a common genetic platform for
the carriage of this gene in Brazil. Inference of phylogenetic orthology of strain Lemef23 and other clinical
isolates suggested an anthropogenic origin.
Conclusions: The findings of this study support the role of flies as vectors of MDR bacteria of clinical
importance and provide the first record of blaNDM-1 and blaCTXM-15 in a Brazilian isolate of K.
quasipneumoniae subsp. similipneumoniae, demonstrating the value of surveying insects as reservoirs of
antibiotic resistance. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Entomologia Médica e Forense. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Biologia. Centro de Ciências da Saúde. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Universidade Federal Fluminense. Faculdade de Medicina. Departamento de Patologia. Niterói, RJ, Brasil. | pt_BR |
Affilliation | Universidade Federal do Rio de Janeiro. Centro de Ciências da Saúde. Instituto de Microbiologia Paulo de Góes. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Universidade Federal do Rio de Janeiro. Centro de Ciências da Saúde. Instituto de Microbiologia Paulo de Góes. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Entomologia Médica e Forense. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Desenvolvimento Tecnológico em Saúde (CDTS). Laboratório de Bioquímica de Proteínas e Peptídeos. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Computacional e Sistemas. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Computacional e Sistemas. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Entomologia Médica e Forense. Rio de Janeiro, RJ, Brasil. | pt_BR |
Subject | Klebsiella quasipneumoniae | pt_BR |
Subject | Flies | pt_BR |
Subject | Public health | pt_BR |
Subject | Antibiotic resistance | pt_BR |
xmlui.metadata.dc.subject.ods | 03 Saúde e Bem-Estar | |