Author | Probst, Christian Macagnan | |
Author | Melo, Myllena de Fátima Alheiros Dias | |
Author | Pavoni, Daniela Parada | |
Author | Toledo, Max Jean de Ornelas | |
Author | Galdino, Tainah Silva | |
Author | Brandão, Adeilton Alves | |
Author | Britto, Constança | |
Author | Krieger, Marco Aurélio | |
Access date | 2021-09-02T14:17:59Z | |
Available date | 2021-09-02T14:17:59Z | |
Document date | 2021 | |
Citation | PROBST, Christian Macagnan et al. A new Trypanosoma cruzi genotyping method enables high resolution evolutionary analyses. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 116, e200538, 12 p, 2021. | pt_BR |
ISSN | 0074-0276 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/48913 | |
Language | eng | pt_BR |
Publisher | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | Trypanosoma cruzi | pt_BR |
Subject in Portuguese | Técnicas de genotipagem | pt_BR |
Subject in Portuguese | Sequenciamento de nucleotídeos de alto rendimento | pt_BR |
Subject in Portuguese | Trans-sialidase | pt_BR |
Title | A new Trypanosoma cruzi genotyping method enables high resolution evolutionary analyses | pt_BR |
Type | Article | pt_BR |
DOI | 10.1590/0074-02760200538 | |
Abstract | BACKGROUND Trypanosoma cruzi is an important human pathogen in Latin America with nearly seven million people
infected. It has a large degree of genetic diversity, classified into six discrete typing units (DTUs), which probably influences
its physiological behavior and clinical manifestations. Several genotyping methods are available, with distinct performance on
easiness, cost, resolution and applicability; no method excels in all parameters.
OBJECTIVES AND METHODS To devise a molecular method for T. cruzi genotyping, based on polymerase chain reaction (PCR)
amplification of a single target with multiple copies in the nuclear genome by large scale sequencing. We have applied this
method to 29 T. cruzi isolates, comprising all described DTUs.
FINDINGS We were able to classify all samples into sub DTU level with high robustness. Evolutionary relationship between DTUs
were ascertained, suggesting that TcIII and TcIV DTUs are non-hybrid, and DTU IV is more similar to the common ancestral.
CONCLUSION As the TS-LSS method is based on a single PCR reaction, comprising several copies of the target, it is probably
useful for clinical samples, when the amount of DNA is a limiting factor. As large scale sequencing systems become more
common, the TS-LSS method can be increasingly applied for T. cruzi genotyping. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Genômica Funcional. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Genômica Funcional. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Universidade Estadual de Maringá. Departamento de Ciências da Saúde. Laboratório de Doença de Chagas. Maringá, PR, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Pesquisas Médicas. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Pesquisas Médicas. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Genômica Funcional. Curitiba, PR, Brasil. | pt_BR |
Subject | Trypanosoma cruzi | pt_BR |
Subject | Genotyping techniques | pt_BR |
Subject | High-throughput nucleotide sequencing | pt_BR |
Subject | Trans-sialidase | pt_BR |
e-ISSN | 1678-8060 | |