Author | Conzentino, Marcelo S. | |
Author | Santos, Tatielle P.C. | |
Author | Selim, Khaled A. | |
Author | Wagner, Berenike | |
Author | Alford, Janette T. | |
Author | Deobald, Nelli | |
Author | Paula, Nigela M. | |
Author | Rego, Fabiane G. M. | |
Author | Zanette, Dalila Lucíola | |
Author | Aoki, Mateus Nóbrega | |
Author | Nardin, Jeanine M. | |
Author | Huergo, Maria C. C. | |
Author | Reis, Rodrigo A. | |
Author | Huergo, Luciano F. | |
Access date | 2021-10-11T17:39:33Z | |
Available date | 2021-10-11T17:39:33Z | |
Document date | 2021 | |
Citation | CONZENTINO, Marcelo S. et al. Ultra-fast, high throughput and inexpensive detection of SARS-CoV-2 seroconversion using Ni2+ magnetic beads. Analytical Biochemistry, v.631, n. 114360, p. 1-6, 2021. | pt_BR |
ISSN | 0003-2697 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/49337 | |
Language | por | pt_BR |
Publisher | Elsevier | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | Magnetic ELISA | pt_BR |
Subject in Portuguese | SARS-CoV-2 | pt_BR |
Title | Ultra-fast, high throughput and inexpensive detection of SARS-CoV-2 seroconversion using Ni2+ magnetic beads | pt_BR |
Type | Article | pt_BR |
DOI | 10.1016/j.ab.2021.114360 | |
Abstract | To monitor the levels of protecting antibodies raised in the population in response to infection and/or to immunization with SARS-CoV-2, we need a technique that allows high throughput and low-cost quantitative
analysis of human IgG antibodies reactive against viral antigens. Here we describe an ultra-fast, high throughput and inexpensive assay to detect SARS-CoV-2 seroconversion in humans. The assay is based on Ni2+ magnetic particles coated with His tagged SARS-CoV-2 antigens. A simple and inexpensive 96 well plate magnetic extraction/homogenization process is described which allows the simultaneous analysis of 96 samples and delivers results in 7 min with high accuracy. | pt_BR |
Affilliation | Universidade Federal do Paraná. Setor Litoral. Matinhos, PR, Brasil. | pt_BR |
Affilliation | Universidade Federal do Paraná. Setor Litoral. Matinhos, PR, Brasil. | pt_BR |
Affilliation | Organismic Interactions Department. Interfaculty Institute for Microbiology and Infection Medicine. Cluster of Excellence ‘Controlling Microbes to Fight Infections’. Tübingen University, Tübingen, Germany. | pt_BR |
Affilliation | Organismic Interactions Department. Interfaculty Institute for Microbiology and Infection Medicine. Cluster of Excellence ‘Controlling Microbes to Fight Infections’. Tübingen University, Tübingen, Germany. | pt_BR |
Affilliation | Organismic Interactions Department. Interfaculty Institute for Microbiology and Infection Medicine. Cluster of Excellence ‘Controlling Microbes to Fight Infections’. Tübingen University, Tübingen, Germany. | pt_BR |
Affilliation | Organismic Interactions Department. Interfaculty Institute for Microbiology and Infection Medicine. Cluster of Excellence ‘Controlling Microbes to Fight Infections’. Tübingen University, Tübingen, Germany. | pt_BR |
Affilliation | Universidade Federal do Paraná. Setor Litoral. Matinhos, PR, Brasil. | pt_BR |
Affilliation | Universidade Federal do Paraná. Programa de Pós-Graduação em Ciências Farmacêuticas. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Hospital Erasto Gaertner. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Universidade Federal do Paraná. Setor Litoral. Matinhos, PR, Brasil. | pt_BR |
Affilliation | Universidade Federal do Paraná. Departamento de Biologia Celular. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Universidade Federal do Paraná. Setor Litoral. Matinhos, PR, Brasil. | pt_BR |
Subject | Enzyme-Linked Immunosorbent Assay | pt_BR |
Subject | Magnetic beads | pt_BR |
Subject | High-Throughput Screening Assays | pt_BR |
Subject in Spanish | Ensayo de Inmunoadsorción Enzimática | pt_BR |
Subject in Spanish | Ensayos Analíticos de Alto Rendimiento | pt_BR |
Subject in French | Test ELISA | pt_BR |
Subject in French | Tests de criblage à haut débit | pt_BR |
DeCS | COVID-19 | pt_BR |
DeCS | Ensaio de Imunoadsorção Enzimática | pt_BR |
DeCS | Ensaios de Triagem em Larga Escala | pt_BR |