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https://www.arca.fiocruz.br/handle/icict/49661
Tipo de documento
ArtigoDireito Autoral
Acesso restrito
Data de embargo
2023
Coleções
- IOC - Artigos de Periódicos [12490]
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DENDRITIC CELLS DEVELOPMENT INTO OSTEOCLAST-TYPE APCS BY 4T1 BREAST TUMOR T CELLS MILIEU BOOST BONE CONSUMPTION
Células dendríticas
Células T
Osteoclastos
Tumor de mama
Câncer de mama e nicho pré-metastático
Dendritic cells
T cells
Osteoclasts
Breast tumor
Breast cancer and pre-metastatic niche
Autor(es)
Afiliação
Universidade Federal Fluminense. Instituto de Biologia. Departamento de Imunobiologia. Laboratório de Imunologia Osteo e Tumoral. Niterói, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ, Brasil / Ministério da Ciência, Tecnologia e Inovações. Conselho Nacional de Desenvolvimento Científico e Tecnológico. Instituto Nacional de Ciência e Tecnologia em Neuroimunomodulação. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rede de Pesquisa em Neuroinflamação. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ, Brasil / Ministério da Ciência, Tecnologia e Inovações. Conselho Nacional de Desenvolvimento Científico e Tecnológico. Instituto Nacional de Ciência e Tecnologia em Neuroimunomodulação. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rede de Pesquisa em Neuroinflamação. Rio de Janeiro, RJ, Brasil.
Resumo em Inglês
Bone metastases occur in 70% of patients with advanced breast cancer, causing severe morbidity and increased mortality due to osteolytic lesions driven by osteoclasts (OCs) inside the bone marrow (BM) microenvironment. A reciprocal vicious cycle between bone remodeling system and the tumor itself is established by the release of growth factors stored in the mineralized matrix, which in turn feed the tumor, changing tumor behavior and growth. However, BM is not a passive host microenvironment for circulating tumor cells, but instead can be actively modified by the primary tumor before metastatic spread occurs. Indeed, we have shown that T cells specific for the 4T1 mammary carcinoma cell line, are characteristically RANKL+ IL-17F+ CD4+ T cells. Those cells arrive in the BM before metastatic cells and set the pre-metastatic niche. In the absence of T cell derived RANKL, there is no pre-metastatic osteolytic disease and bone metastases do not take place. Recently, dendritic cells (DCs), the main T cell partner at the beginning of the immune response, came into the spotlight as a potential source of OCs progenitors under inflammatory conditions. Regarding bone metastasis, nothing is currently known about DCs plasticity or even its partnership with tumor induced T cells for BM pre-metastatic niche formation. Here, we show that splenic CD11c+ DCs stimulated with 4T1 conditioned media (CM) efficiently differentiated into mature and activated multinucleated giant cells (DC-OC) expressing TRAP and IL-23 cytokine. More important, 4T1 CM derived DC-OCs build a positive loop which amplifies the osteolytic phenomena by maintaining the RANKL+ Th17 T cells and by its own osteoclastic activity. In conclusion, our results indicate that differentiation of OCs from DCs may be achievable in the bone pre osteolytic disease context representing an alternative OC differentiation pathway. Besides being induced by high levels of T cells pro osteoclastogenic cytokines, especially by RANKL, DC-OC keep a positive feedback loop towards osteolysis, maintaining the proosteoclastogenic T cell phenotype in the BM.
Palavras-chave
Metástase ósseaCélulas dendríticas
Células T
Osteoclastos
Tumor de mama
Câncer de mama e nicho pré-metastático
Palavras-chave em inglês
Bone metastasisDendritic cells
T cells
Osteoclasts
Breast tumor
Breast cancer and pre-metastatic niche
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