Author | Zamith-Miranda, Daniel | |
Author | Amatuzzi, Rafaela Ferreira | |
Author | Rocha, Isadora Filipaki Munhoz da | |
Author | Martins, Sharon de Toledo | |
Author | Lucena, Aline Castro Rodrigues | |
Author | Vieira, Alexandre Zanatta | |
Author | Trentin, Gabriel | |
Author | Almeida, Fausto | |
Author | Rodrigues, Marcio Lourenço | |
Author | Nakayasu, Ernesto S. | |
Author | Nosanchuk, Joshua D. | |
Author | Alves, Lysangela Ronalte | |
Access date | 2021-11-23T17:24:49Z | |
Available date | 2021-11-23T17:24:49Z | |
Document date | 2021 | |
Citation | ZAMITH-MIRANDA, Daniel de et al. Transcriptional and translational landscape of Candida auris in response to caspofungin. Computational and Structural Biotechnology Journal. n. 19, p. 5264–5277, 2021. | pt_BR |
ISSN | 2001-0370 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/49975 | |
Language | por | pt_BR |
Publisher | Elsevier | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | Candida auris | pt_BR |
Title | Transcriptional and translational landscape of Candida auris in response to caspofungin | pt_BR |
Type | Article | pt_BR |
DOI | 10.1016/j.csbj.2021.09.007 | |
Abstract | Candida auris has emerged as a serious worldwide threat by causing opportunistic infections that are frequently resistant to one or more conventional antifungal medications resulting in high mortality rates.
Against this backdrop, health warnings around the world have focused efforts on understanding C. auris
fungal biology and effective prevention and treatment approaches to combat this fungus. To date, there is
little information about the differentially expressed genes when this fungus is treated with conventional
antifungals, and caspofungin is a standard echinocandin deployed in the therapy against C. auris. In this
work, we treated two distinct strains of C. auris for 24 h with caspofungin, and the cellular responses were
evaluated at the morphological, translational and transcriptional levels. We first observed that the echinocandin caused morphological alterations, aggregation of yeast cells, and modifications in the cell wall composition of C. auris. Transcriptomic analysis revealed an upregulation of genes related to the synthesis of the cell wall, ribosome, and cell cycle after exposure to caspofungin. Supporting these findings, the integrated proteomic analysis showed that caspofungin-treated cells were enriched in ribosomerelated proteins and cell wall, especially mannoproteins. Altogether, these results provide further insights into the biology of C. auris and expands our understanding regarding the antifungal activity of caspofungin and reveal cellular targets, as the mannose metabolism, that can be further explored for the development of novel antifungals. | pt_BR |
Affilliation | Department of Microbiology and Immunology. Albert Einstein College of Medicine. Bronx, New York, USA. / Division of Infectious Diseases. Department of Medicine. Albert Einstein College of Medicine. Bronx, New York, USA. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Regulação da Expressão Gênica. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Regulação da Expressão Gênica. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Regulação da Expressão Gênica. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Ciências e Tecnologias Aplicadas em Saúde. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Ciências e Tecnologias Aplicadas em Saúde. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Departamento de Bioquímica e Imunologia. Ribeirão Preto, SP, Brasil. | pt_BR |
Affilliation | Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Departamento de Bioquímica e Imunologia. Ribeirão Preto, SP, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Regulação da Expressão Gênica. Curitiba, PR, Brasil. / Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Pacific Northwest National Laboratory. Richland, WA, USA. | pt_BR |
Affilliation | Department of Microbiology and Immunology. Albert Einstein College of Medicine. Bronx, New York, USA. / Division of Infectious Diseases. Department of Medicine. Albert Einstein College of Medicine. Bronx, New York, USA. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Regulação da Expressão Gênica. Curitiba, PR, Brasil. | pt_BR |
Subject | Transcriptomic | pt_BR |
Subject | Proteomics | pt_BR |
Subject | Drug Resistance | pt_BR |
Subject | Metabolic Stress Response | pt_BR |
Subject in Spanish | Transcriptoma | pt_BR |
Subject in Spanish | Proteómica | pt_BR |
Subject in Spanish | Resistencia a Medicamentos | pt_BR |
Subject in Spanish | Estrés Fisiológico | pt_BR |
Subject in French | Transcriptome | pt_BR |
Subject in French | Protéomique | pt_BR |
Subject in French | Résistance aux substances | pt_BR |
Subject in French | Stress physiologique | pt_BR |
DeCS | Candida | pt_BR |
DeCS | Transcriptoma | pt_BR |
DeCS | Proteômica | pt_BR |
DeCS | Resistência a Medicamentos | pt_BR |
DeCS | Estresse Fisiológico | pt_BR |
DeCS | Farmacorresistência Fúngica | pt_BR |