Author | Luz, Yasmin da Silva | |
Author | Paixão, Amanda R. | |
Author | Bernardes, Carla Polyana O. S. | |
Author | Silva, Thaílla Souza da | |
Author | Tavares, Natalia Machado | |
Author | Brodskyn, Claudia Ida | |
Author | Veras, Patricia Sampaio Tavares | |
Author | Menezes, Juliana Pereira Bezerra de | |
Access date | 2021-12-03T17:08:13Z | |
Available date | 2021-12-03T17:08:13Z | |
Document date | 2021 | |
Citation | LUZ, Yasmin da Silva et al. Cell Migration and Cell Adhesion Assays to Investigate Leishmania-Host Cell Interaction. Journal of Visualized Experiments, 2021. | pt_BR |
ISSN | 1940-087X | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/50158 | |
Sponsorship | Bahia Research Support
Foundation (Fapesb), grant number 9092/2015. The authors
acknowledge CNPq, Capes and Fapesb for financial support
via scholarships. | pt_BR |
Language | eng | pt_BR |
Publisher | MyJove Corporation | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | Leishmania | pt_BR |
Subject in Portuguese | Células Dendríticas | pt_BR |
Subject in Portuguese | Macrófagos | pt_BR |
Subject in Portuguese | Adesão Celular | pt_BR |
Title | Cell Migration and Cell Adhesion Assays to Investigate Leishmania-Host Cell Interaction | pt_BR |
Type | Article | pt_BR |
Abstract | Leishmania is an intracellular protozoan parasite that causes a broad spectrum
of clinical manifestations, ranging from self-resolving localized cutaneous lesions
to a highly fatal visceral form of the disease. An estimated 12 million people
worldwide are currently infected, and another 350 million face risk of infection. It is
known that host cells infected by Leishmania parasites, such as macrophages or
dendritic cells, can migrate to different host tissues, yet how migration contributes to
parasite dissemination and homing remains poorly understood. Therefore, assessing
these parasites' ability to modulate host cell response, adhesion, and migration will
shed light on mechanisms involved in disease dissemination and visceralization.
Cellular migration is a complex process in which cells undergo polarization and
protrusion, allowing them to migrate. This process, regulated by actin and tubulinbased
microtubule dynamics, involves different factors, including the modulation of
cellular adhesion to the substrate. Cellular adhesion and migration processes have
been investigated using several models. Here, we describe a method to characterize
the migratory aspects of host cells during Leishmania infection. This detailed protocol
presents the differentiation and infection of dendritic cells, the analysis of host cell
motility and migration, and the formation of adhesion complexes and actin dynamics.
This in vitro protocol aims to further elucidate mechanisms involved in Leishmania
dissemination within vertebrate host tissues and can also be modified and applied to
other cell migration studies. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Hospedeiro - Interação Parasitária e Epidemiologia. Salvador, BA, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Hospedeiro - Interação Parasitária e Epidemiologia. Salvador, BA, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Hospedeiro - Interação Parasitária e Epidemiologia. Salvador, BA, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Hospedeiro - Interação Parasitária e Epidemiologia. Salvador, BA, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Hospedeiro - Interação Parasitária e Epidemiologia. Salvador, BA, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Hospedeiro - Interação Parasitária e Epidemiologia. Salvador, BA, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Hospedeiro - Interação Parasitária e Epidemiologia. Salvador, BA, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Hospedeiro - Interação Parasitária e Epidemiologia. Salvador, BA, Brasil. | pt_BR |
Subject | Leishmaniasis | pt_BR |
Subject | Dendritic cells | pt_BR |
Subject | Macrophages | pt_BR |
Subject | Cell adhesion | pt_BR |
e-ISSN | 10.3791/62461 (2021). | |