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https://www.arca.fiocruz.br/handle/icict/50190
ESSENTIAL OIL FROM BARK OF ANIBA PARVIFLORA (MEISN.) MEZ (LAURACEAE) REDUCES HEPG2 CELL PROLIFERATION AND INHIBITS TUMOR DEVELOPMENT IN A XENOGRAFT MODEL
Author
Oliveira, Felipe P. de
Rodrigues, Ana Carolina B. da C.
Lima, Emilly J. S. P. de
Silva, Valdenizia R.
Santos, Luciano de S.
Anunciação, Talita A. da
Nogueira, Mateus L.
Soares, Milena Botelho Pereira
Dias, Rosane B.
Rocha, Clarissa Araujo Gurgel
Duvoisin Junior, Sérgio
Albuquerque, Patrícia M.
Lima, Emerson S.
Gonçalves, José F. C.
Bataglion, Giovana A.
Costa, Emmanoel V.
Silva, Felipe M. A. da
Koolen, Hector H. F.
Bezerra, Daniel Pereira
Rodrigues, Ana Carolina B. da C.
Lima, Emilly J. S. P. de
Silva, Valdenizia R.
Santos, Luciano de S.
Anunciação, Talita A. da
Nogueira, Mateus L.
Soares, Milena Botelho Pereira
Dias, Rosane B.
Rocha, Clarissa Araujo Gurgel
Duvoisin Junior, Sérgio
Albuquerque, Patrícia M.
Lima, Emerson S.
Gonçalves, José F. C.
Bataglion, Giovana A.
Costa, Emmanoel V.
Silva, Felipe M. A. da
Koolen, Hector H. F.
Bezerra, Daniel Pereira
Affilliation
"Múltipla ver em Notas"
Abstract
Aniba parviflora (MEISN.) MEZ (Lauraceae) is an aromatic plant of the Amazon rainforest, which has a
tremendous commercial value in the perfumery industry; it is popularly used as flavoring sachets and aromatic
baths. In Brazilian folk medicine, A. parviflora is used to treat victims of snakebites. Herein, we analyzed the
chemical composition of A. parviflora bark essential oil (EO) and its effect on the growth of human hepatocellular
carcinoma HepG2 cells in vitro and in vivo. EO was obtained by hydrodistillation and characterized by GC-MS and
GC-FID. The main constituents of EO were linalool (16.3:3.15), α-humulene (14.5:2.41 %), δ-cadinene (10.2:
1.09 %), α-copaene (9.51:1.12%) and germacrene B (7.58:2.15 %). Initially, EO’s cytotoxic effect was evaluated
against five cancer cell lines (HepG2, MCF-7, HCT116, HL-60 and B16-F10) and one non-cancerous one (MRC-5),
using the Alamar blue method after 72 h of treatment. The calculated IC50 values were 9.05, 22.04, >50, 15.36,
17.57, and 30.46 μg/mL, respectively. The best selectivity was for HepG2 cells with a selective index of 3.4. DNA
Fragmentation and cell cycle distribution were quantified in HepG2 cells by flow cytometry after a treatment
period of 24 and 48 h. The effect of EO on tumor development in vivo was evaluated in a xenograft model using
C.B-17 SCID mice engrafted with HepG2 cells. In vivo tumor growth inhibition of HepG2 xenograft at the doses of
40 and 80 mg/kg were 12.1 and 62.4 %, respectively.
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