Author | Cunha, Mylena Lemes | |
Author | Silva, Stella Schuster da | |
Author | Stracke, Mateus Cassaboni | |
Author | Zanette, Dalila Lucíola | |
Author | Aoki, Mateus Nóbrega | |
Author | Blanes, Lucas | |
Access date | 2022-03-24T14:58:45Z | |
Available date | 2022-03-24T14:58:45Z | |
Document date | 2022 | |
Citation | CUNHA, Mylena Lemes et al. Sample Preparation for Lab-on-a-Chip Systems in Molecular Diagnosis: A Review. Analytical Chemistry, n. 94, p. 41−58, 2022. | pt_BR |
ISSN | 1520-6882 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/51855 | |
Language | por | pt_BR |
Publisher | American Chemical Society | pt_BR |
Rights | open access | pt_BR |
Title | Sample preparation for lab-on-a-chip systems in molecular diagnosis: a review | pt_BR |
Type | Article | pt_BR |
DOI | 10.1021/acs.analchem.1c04460 | |
Abstract | Rapid and low-cost molecular analysis is especially required for early and specific diagnostics, quick decision-making, and sparing patients from unnecessary tests and hospitals from extra costs. One way to achieve this objective is through automated molecular diagnostic devices. Thus, sample-to-answer microfluidic devices are emerging with the promise of delivering a complete molecular diagnosis system that includes nucleic acid extraction, amplification, and detection steps in a single device. The biggest issue in such equipment is the extraction process, which is normally laborious and time-consuming but extremely important for sensitive and specific detection. Therefore, this Review focuses on automated or semiautomated extraction methodologies used in lab-on-a-chip devices. More than 15 different extraction methods developed over the past 10 years have been analyzed in terms of their advantages and disadvantages to improve extraction procedures in future studies. Herein, we are able to explain the high applicability of the extraction methodologies due to the large variety of samples in which different techniques were employed, showing that their applications are not limited to medical diagnosis. Moreover, we are able to conclude that further research in the field would be beneficial because the methodologies presented can be affordable, portable, time efficient, and easily manipulated, all
of which are strong qualities for point-of-care technologies. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Ciências e Tecnologias Aplicadas em Saúde. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Ciências e Tecnologias Aplicadas em Saúde. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Ciências e Tecnologias Aplicadas em Saúde. Curitiba, PR, Brasil / Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Ciências e Tecnologias Aplicadas em Saúde. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Ciências e Tecnologias Aplicadas em Saúde. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Ciências e Tecnologias Aplicadas em Saúde. Curitiba, PR, Brasil / Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil. | pt_BR |
Subject | Molecular Diagnostic Testing | pt_BR |
Subject | Lab-On-A-Chip Devices | pt_BR |
Subject | Analytic Sample Preparation Methods | pt_BR |
Subject | Point-of-Care Systems | pt_BR |
Subject in Spanish | Técnicas de Diagnóstico Molecular | pt_BR |
Subject in Spanish | Dispositivos Laboratorio en un Chip | pt_BR |
Subject in Spanish | Métodos Analíticos de la Preparación de la Muestra | pt_BR |
Subject in Spanish | Sistemas de Atención de Punto | pt_BR |
Subject in French | Techniques de diagnostic moléculaire | pt_BR |
Subject in French | Laboratoires sur puces | pt_BR |
Subject in French | Méthodes de préparation d'échantillons analytiques | pt_BR |
Subject in French | Systèmes automatisés lit malade | pt_BR |
DeCS | Teste de Diagnóstico Molecular | pt_BR |
DeCS | Dispositivos Analíticos em Microchips | pt_BR |
DeCS | Métodos Analíticos de Preparação de Amostras | pt_BR |
DeCS | Sistemas Automatizados de Assistência Junto ao Leito | pt_BR |