Author | Inoue, Alexandre Haruo | |
Author | Domingues, Patricia Ferreira | |
Author | Serpeloni, Mariana | |
Author | Hiraiwa, Priscila Mazzocchi | |
Author | Vidal, Newton Medeiros | |
Author | Butterfield, Erin R. | |
Author | Pino, Ricardo Canavate del | |
Author | Ludwig, Adriana | |
Author | Boehm, Cordula | |
Author | Field, Mark C. | |
Author | Ávila, Andréa Rodrigues | |
Access date | 2022-05-02T18:59:08Z | |
Available date | 2022-05-02T18:59:08Z | |
Document date | 2022 | |
Citation | INOUE, Alexandre Haruo et al. Proteomics uncovers novel components of na interactive protein network supporting RNA export in trypanosomes. MCP Research, v. 21 n. 3, p. 1–24, Mar. 2022. | pt_BR |
ISSN | 1535-9484 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/52506 | |
Language | por | pt_BR |
Publisher | American Society for Biochemistry and Molecular Biology | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | Trypanosoma cruzi | pt_BR |
Subject in Portuguese | Trypanosoma | pt_BR |
Subject in Portuguese | Cinetoplastídeos | pt_BR |
Title | Proteomics uncovers novel components of na interactive protein network supporting RNA export in trypanosomes | pt_BR |
Type | Article | pt_BR |
DOI | 10.1016/j.mcpro.2022.100208 | |
Abstract | In trypanosomatids, transcription is polycistronic and all mRNAs are processed by trans-splicing, with export mediated by noncanonical mechanisms. Although mRNA export is central to gene regulation and expression, few orthologs of proteins involved in mRNA export in higher eukaryotes are detectable in trypanosome genomes, necessitating direct identification of protein components. We previously described conserved mRNA export pathway components in Trypanosoma cruzi, including orthologs of Sub2, a component of the TREX complex, and eIF4AIII (previously Hel45), a core component of the exon junction complex (EJC). Here, we searched for protein interactors of both proteins using cryomilling and mass spectrometry. Significant overlap between TcSub2 and TceIF4AIII-interacting protein cohorts suggests that both proteins associate with similar machinery. We identified several interactions with conserved core components of the EJC and multiple additional complexes, together with proteins specific to trypanosomatids. Additional immunoisolations of kinetoplastid-specific proteins both validated and extended the superinteractome, which is capable of supporting RNA processing from splicing through to nuclear export and cytoplasmic events. We also suggest that only proteomics is powerful enough to uncover the high connectivity between multiple aspects of mRNA metabolism and to uncover kinetoplastid-specific components that create a unique amalgam to support trypanosome mRNA maturation. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. | pt_BR |
Affilliation | National Center for Biotechnology Information. National Library of Medicine. National Institutes of Health. Bethesda, Maryland, USA. | pt_BR |
Affilliation | School of Life Sciences. University of Dundee. Dundee, Scotland, UK. | pt_BR |
Affilliation | School of Life Sciences. University of Dundee. Dundee, Scotland, UK. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. | pt_BR |
Affilliation | School of Life Sciences. University of Dundee. Dundee, Scotland, UK. | pt_BR |
Affilliation | School of Life Sciences. University of Dundee. Dundee, Scotland, UK. / Biology Centre. University of South Bohemia. Ceské Budejovice, Czech Republic. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. | pt_BR |
Subject | Proteomics | pt_BR |
Subject | RNA Messenger | pt_BR |
Subject | RNA Splicing | pt_BR |
Subject in Spanish | Proteómica | pt_BR |
Subject in Spanish | ARN Mensajero | pt_BR |
Subject in Spanish | Empalme del ARN | pt_BR |
Subject in French | Protéomique | pt_BR |
Subject in French | ARN messager | pt_BR |
Subject in French | Épissage des ARN | pt_BR |
DeCS | Proteômica | pt_BR |
DeCS | RNA Mensageiro | pt_BR |
DeCS | Splicing de RNA | pt_BR |